Marine Collagen Substrates for 2D and 3D Ovarian Cancer Cell Systems
| Autor: | Deyarina Gonzalez, Francesca Paradiso, Joan Fitzgerald, Lewis Francis, Francesca Taraballi, R. Steven Conlan, Frank Barry, Seydou Yao |
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| Rok vydání: | 2019 |
| Předmět: |
collagen
0301 basic medicine Histology lcsh:Biotechnology Cell Biomedical Engineering Bioengineering 02 engineering and technology Rhizostoma pulmo Extracellular matrix 03 medical and health sciences 3D cell culture biocompatibility lcsh:TP248.13-248.65 medicine Epithelial–mesenchymal transition Amino acid content Original Research cell culture biology Chemistry Bioengineering and Biotechnology jellyfish 021001 nanoscience & nanotechnology medicine.disease biology.organism_classification Cell biology ovarian cancer 030104 developmental biology medicine.anatomical_structure Cell culture 0210 nano-technology Ovarian cancer Biotechnology |
| Zdroj: | Frontiers in Bioengineering and Biotechnology, Vol 7 (2019) Frontiers in Bioengineering and Biotechnology |
| ISSN: | 2296-4185 |
| DOI: | 10.3389/fbioe.2019.00343 |
| Popis: | A fundamental structural component of extracellular matrix in all connective and interstitial tissue, collagen is the most abundant protein in the human body. To date, mammalian collagens sources represent the golden standard for multiple biomedical applications, while marine-derived collagens have largely been used in industry (food, pharmaceutical, and cosmetic), with little use in research and clinical applications. Herein we demonstrate the effective use Rhizostoma pulmo jellyfish collagen, a source of biocompatible, sustainable collagen for 2D and 3D cell culture, addressing the global drive for technological developments that result in the replacement of animals and their derived products in research. Jellyfish collagen harbors similar structural features mammalian collagen type I, despite differing slightly in amino acid content. Jellyfish collagen supports ovarian cancer (OvCa) cell line proliferation, cellular morphology and expression of epithelial to mesenchymal transition markers, supporting the use of R. pulmo as a non-mammalian collagen cell culture substrate. Furthermore, R. pulmo collagen is effective in 3D device fabrication such as sponges where it mimics tissue architecture complexity. OvCa cells migrated and differentiated within the R. pulmo collagen 3D scaffolds confirming its suitability for advanced cell culturing applications, providing an excellent alternative to mammalian collagen sources for the culture of human cells. |
| Databáze: | OpenAIRE |
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