Abdominal Obesity-Related Disturbance of Insulin Sensitivity Is Associated with CD8+ EMRA Cells in the Elderly
| Autor: | Boßlau, Tim K., Wasserfurth, Paulina, Krüger, Britta, Reichel, Thoma, Palmowski, Jana, Nebl, Josefine, Weyh, Christopher, Schenk, Alexander, Joisten, Niklas, Stahl, Frank, Thoms, Stefanie, Gebhardt, Kristina, Hahn, Andreas, Krüger, Karsten |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
obesity interleukin 1beta Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften Biologie peripheral blood mononuclear cell Cytomegalovirus CD8-Positive T-Lymphocytes Body Mass Index human experiment immunology Elderly homeostasis model assessment insulin resistance middle aged cytokine receptor type tyrosine protein phosphatase C glucose CD8+ T lymphocyte Biology (General) hemoglobin A1c pathophysiology Kynurenine T lymphocyte subpopulation adult Tryptophan biological marker waist circumference female waist hip ratio Obesity Abdominal Cytokines blood sampling kynurenine pathway insulin QH301-705.5 tumor necrosis factor glucose metabolism adipocytokine interleukin 6 elderly Article abdominal obesity immunological memory body weight HOMA index Adipokines fractalkine blood ddc:570 cross-sectional study insulin sensitivity Humans Obesity human normal human Kynurenine pathway tryptophan metabolism resistin hip circumference body composition adiponectin flow cytometry case control study major clinical study body mass T-EMRA cells enzyme linked immunosorbent assay Cross-Sectional Studies Case-Control Studies interleukin 1 receptor blocking agent Leukocyte Common Antigens T‐EMRA cells metabolism Immunologic Memory interleukin 15 Biomarkers |
| Zdroj: | Cells Volume 10 Issue 5 Cells, Vol 10, Iss 998, p 998 (2021) Cells 10 (2021), Nr. 5 |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells10050998 |
| Popis: | Aging and overweight increase the risk of developing type 2 diabetes mellitus. In this cross‐sectional study, we aimed to investigate the potential mediating role of T‐EMRA cells and inflammatory markers in the development of a decreased insulin sensitivity. A total of 134 healthy older volunteers were recruited (age 59.2 (SD 5.6) years). T cell subpopulations were analyzed by flow cytometry. Furthermore, body composition, HOMA‐IR, plasma tryptophan (Trp) metabolites, as well as cytokines and adipokines were determined. Using subgroup and covariance analyses, the influence of BMI on the parameters was evaluated. Moreover, correlation, multiple regression, and mediation analyses were performed. In the subgroup of participants with obesity, an increased proportion of CD8+EMRA cells and elevated concentrations of plasma kynurenine (KYN) were found compared to the lower‐weight subgroups. Linear regression analysis revealed that an elevated HOMA‐IR could be predicted by a higher proportion of CD8+EMRA cells and KYN levels. A mediation analysis showed a robust indirect effect of the Waist‐to‐hip ratio on HOMA‐IR mediated by CD8+EMRA cells. Thus, the deleterious effects of abdominal obesity on glucose metabolism might be mediated by CD8+EMRA cells in the elderly. Longitudinal studies should validate this assumption and analyze the suitability of CD8+EMRA cells as early predictors of incipient prediabetes. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
| Databáze: | OpenAIRE |
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