Hen1 is required for oocyte development and piRNA stability in zebrafish

Autor: René F. Ketting, Stefan Redl, Eugene Berezikov, Elke F. Roovers, Lucas J.T. Kaaij, Marjo J. den Broeder, Peter Ladurner, Maartje J. Luteijn, Leonie M. Kamminga
Přispěvatelé: Hubrecht Institute for Developmental Biology and Stem Cell Research, Stem Cell Aging Leukemia and Lymphoma (SALL), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
Rok vydání: 2012
Předmět:
Male
Small RNA
Embryo
Nonmammalian
Piwi
piRNA
0302 clinical medicine
Testis
RNA
Small Interfering
Zebrafish
In Situ Hybridization
Genetics
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
Argonaute
Corrigenda
medicine.anatomical_structure
Female
RNA 3' End Processing
Germ cell
Subcellular Fractions
endocrine system
Retroelements
Molecular Sequence Data
Piwi-interacting RNA
Biology
Article
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
medicine
RasiRNA
Animals
Immunoprecipitation
Amino Acid Sequence
RNA
Messenger
Uridine
Hen1
Molecular Biology
030304 developmental biology
Sequence Homology
Amino Acid
General Immunology and Microbiology
urogenital system
RNA
Methyltransferases
Zebrafish Proteins
Oocyte
biology.organism_classification
Blotting
Northern
zebrafish
Mutation
Oocytes
germ line
030217 neurology & neurosurgery
Zdroj: EMBO Journal, 29(21), 3688-3700. Nature Publishing Group
The EMBO Journal
EMBO Journal, 29(21), 3688-3700. Wiley
ISSN: 0261-4189
DOI: 10.1038/emboj.2011.462
Popis: Piwi-interacting RNAs (piRNAs) are germ line-specific small RNA molecules that have a function in genome defence and germ cell development. They associate with a specific class of Argonaute proteins, named Piwi, and function through an RNA interference-like mechanism. piRNAs carry a 2'-O-methyl modification at their 3' end, which is added by the Hen1 enzyme. We show that zebrafish hen1 is specifically expressed in germ cells and is essential for maintaining a female germ line, whereas it is dispensable in the testis. Hen1 protein localizes to nuage through its C-terminal domain, but is not required for nuage formation. In hen1 mutant testes, piRNAs become uridylated and adenylated. Uridylation frequency is highest on retro-transposon-derived piRNAs and is accompanied by decreased piRNA levels and mild derepression of transposon transcripts. Altogether, our data suggest the existence of a uridylation-mediated 3'-5'exonuclease activity acting on piRNAs in zebrafish germ cells, which is counteracted by nuage-bound Hen1 protein. This system discriminates between piRNA targets and is required for ovary development and fully efficient transposon silencing.
Databáze: OpenAIRE
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