Next-generation sequencing for the diagnosis of hepatitis B: current status and future prospects
| Autor: | Francisco Rodríguez-Algarra, Josep Quer, Maria Francesca Cortese, Maria Buti, David Tabernero, Selene Garcia-Garcia, Ariadna Rando-Segura, Francisco Rodriguez-Frias |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Hepatitis B virus Genotype Viral quasispecies Computational biology medicine.disease_cause Genome DNA sequencing Virus Pathology and Forensic Medicine 03 medical and health sciences Liver disease 0302 clinical medicine Genetics medicine Humans Molecular Biology business.industry High-Throughput Nucleotide Sequencing Hepatitis B medicine.disease Quasispecies 030104 developmental biology 030220 oncology & carcinogenesis DNA Viral Molecular Medicine business |
| Zdroj: | Expert review of molecular diagnostics. 21(4) |
| ISSN: | 1744-8352 |
| Popis: | Introduction Hepatitis B virus (HBV) causes a complex and persistent infection with a major impact on patients health. Viral-genome sequencing can provide valuable information for characterizing virus genotype, infection dynamics and drug and vaccine resistance. Areas covered This article reviews the current literature to describe the next-generation sequencing progress that facilitated a more comprehensive study of HBV quasispecies in diagnosis and clinical monitoring. Expert opinion HBV variability plays a key role in liver disease progression and treatment efficacy. Second-generation sequencing improved the sensitivity for detecting and quantifying mutations, mixed genotypes and viral recombination. Third-generation sequencing enables the analysis of the entire HBV genome, although the high error rate limits its use in clinical practice. |
| Databáze: | OpenAIRE |
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