Effects of Cholesterol in Stress-Related Neuronal Death—A Statistical Analysis Perspective

Autor: Farzan Aminian, James L. Roberts, Saber Elaydi, Nikita Viswasam, Kwan H Cheng, George Livadiotis, Maher A. Dayeh
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
medicine.medical_specialty
Cell Survival
medicine.disease_cause
Cellular viability
Catalysis
Article
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Plasma cholesterol
Stress
Physiological
Internal medicine
medicine
oxidative stress
Statistical analysis
Viability assay
Physical and Theoretical Chemistry
Molecular Biology
Lipid raft
lcsh:QH301-705.5
Spectroscopy
Cells
Cultured
Neurons
Membrane cholesterol
Cell Death
Dose-Response Relationship
Drug
Cholesterol
Organic Chemistry
Cell Membrane
General Medicine
Computer Science Applications
030104 developmental biology
Endocrinology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Alzheimer’s disease
030217 neurology & neurosurgery
Oxidative stress
dementia
cellular viability
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 21, Iss 2905, p 2905 (2020)
Volume 21
Issue 8
ISSN: 1422-0067
Popis: The association between plasma cholesterol levels and the development of dementia continues to be an important topic of discussion in the scientific community, while the results in the literature vary significantly. We study the effect of reducing oxidized neuronal cholesterol on the lipid raft structure of plasma membrane. The levels of plasma membrane cholesterol were reduced by treating the intact cells with methyl-ß-cyclodextrin (MßCD). The relationship between the cell viability with varying levels of MßCD was then examined. The viability curves are well described by a modified form of the empirical Gompertz law of mortality. A detailed statistical analysis is performed on the fitting results, showing that increasing MßCD concentration has a minor, rather than significant, effect on the cellular viability. In particular, the dependence of viability on MßCD concentration was found to be characterized by a ~25% increase per 1 μM of MßCD concentration.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje