Hoogsteen-Paired Homopurine [RP-PS]-DNA and Homopyrimidine RNA Strands Form a Thermally Stable Parallel Duplex
| Autor: | Beata Rebowska, Magdalena Janicka, Piotr Guga, Anna Maciaszek, Genowefa Nowak |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Circular dichroism
Base pair Molecular Sequence Data Molecular Conformation Biophysics Analytical chemistry Antiparallel (biochemistry) Cytosine chemistry.chemical_compound Nucleic Acids Base Pairing Base Sequence Circular Dichroism Temperature RNA Hydrogen Bonding DNA Hydrogen-Ion Concentration Deoxyribonucleoside Crystallography Pyrimidines Spectrometry Fluorescence chemistry Purines Duplex (building) Nucleic Acid Conformation |
| Zdroj: | Biophysical Journal. (10):3567-3574 |
| ISSN: | 0006-3495 |
| DOI: | 10.1529/biophysj.107.108183 |
| Popis: | Homopurine deoxyribonucleoside phosphorothioates possessing all internucleotide linkages of RP configuration form a duplex with an RNA or 2′-OMe-RNA strand with Hoogsteen complementarity. The duplexes formed with RNA templates are thermally stable at pH 5.3, while those formed with a 2′-OMe-RNA are stable at neutrality. Melting temperature and fluorescence quenching experiments indicate that the strands are parallel. Remarkably, these duplexes are thermally more stable than parallel Hoogsteen duplexes and antiparallel Watson-Crick duplexes formed by unmodified homopurine DNA molecules of the same sequence with corresponding RNA templates. |
| Databáze: | OpenAIRE |
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