Inhibition of STAT3 Reduces Astrocytoma Cell Invasion and Constitutive Activation of STAT3 Predicts Poor Prognosis in Human Astrocytoma
| Autor: | Jie Ma, Qinchuan Liang, Chenkai Ma, Yang Zhao, Guodong Gao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male STAT3 Transcription Factor Pathology medicine.medical_specialty Cell lcsh:Medicine Apoptosis Disease-Free Survival chemistry.chemical_compound Cell Line Tumor medicine Biomarkers Tumor Cell Adhesion Humans Neoplasm Invasiveness U87 STAT3 Cell adhesion lcsh:Science neoplasms Gene knockdown Multidisciplinary biology Cadherin Brain Neoplasms lcsh:R Astrocytoma Middle Aged medicine.disease Cadherins nervous system diseases Neoplasm Proteins Vascular endothelial growth factor Survival Rate medicine.anatomical_structure chemistry nervous system Matrix Metalloproteinase 9 Cancer research biology.protein Matrix Metalloproteinase 2 lcsh:Q Female Glioblastoma Research Article Follow-Up Studies |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 12, p e84723 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Astrocytoma cells characteristically possess high invasion potentials. Recent studies have revealed that knockdown of signal transducers and activators of transcription 3 (STAT3) expression by RNAi induces apoptosis in astrocytoma cell. Nevertheless, the distinct roles of STAT3 in astrocytoma's invasion and recurrence have not been elucidated. In this study, we silenced STAT3 using Small interfering RNAs in two human glioblastoma multiforme (GBM) cell lines (U251 and U87), and investigated the effect on GBM cell adhesion and invasion. Our results demonstrate that disruption of STAT3 inhibits GBM cell's adhesion and invasion. Knockdown of STAT3 significantly increased E-cadherin but decreased N-cadherin, vascular endothelial growth factor, matrix metalloproteinase 2 and matrix metalloproteinase 9. Additionally, expression of pSTAT3(Tyr705) correlates with astrocytoma WHO classification, Karnofsky performance status scale score, tumor recurrence and survival. Furthermore, pSTAT3(Tyr705) is a significant prognostic factor in astrocytoma. In conclusion, STAT3 may affect astrocytoma invasion, expression of pSTAT3(Tyr705) is a significant prognostic factor in tumor recurrence and overall survival in astrocytoma patients. Therefore, STAT3 may provide a potential target for molecular therapy in human astrocytoma, and pSTAT3(Tyr705)could be an important biomarker for astrocytoma prognosis. |
| Databáze: | OpenAIRE |
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