Ultra-fast quantitation of voriconazole in human plasma by coated blade spray mass spectrometry
| Autor: | Janusz Pawliszyn, Ezel Boyacı, Germán Augusto Gómez-Ríos, Marcos Tascon, Justen J. Poole, Nathaly Reyes-Garcés |
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| Rok vydání: | 2017 |
| Předmět: |
Analyte
Antifungal Agents Calibration curve Clinical Biochemistry Pharmaceutical Science 010402 general chemistry Mass spectrometry 01 natural sciences Vial Mass Spectrometry Analytical Chemistry Pharmacokinetics Tandem Mass Spectrometry Drug Discovery medicine Humans Sample preparation Chromatography High Pressure Liquid Spectroscopy Voriconazole Chromatography Chemistry 010401 analytical chemistry Extraction (chemistry) Reproducibility of Results 0104 chemical sciences Drug Monitoring medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 144:106-111 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2017.03.009 |
| Popis: | Voriconazole is a triazole broad-spectrum antifungal medication often used to treat fungal infections caused by Aspergillus and Fusarium species. One of the main challenges associated with the implementation of this medication is its narrow therapeutic concentration range, demonstrating toxicity at concentrations above 6μg/mL and limited efficacy at concentrations below 2μg/mL. As a result, methodologies which permit the rapid and accurate quantitation of voriconazole in patients are highly desirable. In this work two different approaches based on coated blade spray directly coupled to mass spectrometry (CBS-MS) are introduced; each enabling the quantitation of voriconazole in plasma samples with a simple and fast sample preparation and no chromatographic step. The first approach involves a rapid extraction (1min) of the target analyte from 300μL of human plasma using conventional laboratory vessels (e.g. vial, 96-well plate). Alternatively, the second strategy consists of a 2min extraction from a plasma droplet (10μL) placed on the coated area of the blade. Both procedures were successfully validated and good linearity (R2≥0.998), accuracy (91-122%) and precision ( |
| Databáze: | OpenAIRE |
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