Translocation t(5;14)(q35;q32) in three cases of childhood T cell acute lymphoblastic leukemia: a new recurring and cryptic abnormality
| Autor: | Pierre G. Lutz, Entz-Werlé N, J Aurich Costa, D Eyer, Vincent Leymarie, Michel Lessard, Catherine Helias, Annie Falkenrodt, D Cherif |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Chromosomal translocation Biology Polymerase Chain Reaction Translocation Genetic Chromosome Painting law.invention law Multiplex polymerase chain reaction medicine Humans Leukemia-Lymphoma Adult T-Cell Child Polymerase chain reaction Chromosomes Human Pair 14 Genetics medicine.diagnostic_test Hybridization probe Cytogenetics Karyotype Hematology Gene rearrangement Telomere Molecular biology Phenotype Oncology Child Preschool Karyotyping Chromosomes Human Pair 5 DNA Probes Fluorescence in situ hybridization |
| Zdroj: | Leukemia. 16:7-12 |
| ISSN: | 1476-5551 0887-6924 |
| DOI: | 10.1038/sj.leu.2402347 |
| Popis: | We report three cases of T-ALL in which conventional cytogenetic analysis yielded normal karyotypes, but for which a new M-FISH technique (IPM-FISH) was able to detect a translocation. For these patients this technique highlighted a new, recurring and cryptic translocation t(5;14)(q35;q32) in childhood T-ALL which might be phenotypically restricted. The most innovative part of this technique is the use of interspersed polymerase chain reaction (IRS-PCR) painting probes that show an R-band pattern simultaneous with the combinatorial labeling. Contrary to the DOP-PCR, IRS-PCR-derived probes provide stronger hybridization signals at the telomeric ends that potentially increase the possibility of detecting cryptic translocations. All the IPM-FISH findings were validated by FISH with whole chromosome painting and unique sequence probes. These results demonstrate the efficient use of IPM-FISH as an improved, single-step method for the identification of cryptic chromosomal abnormalities. This new IPM-FISH technique is a good tool to display cryptic chromosomal abnormalities. |
| Databáze: | OpenAIRE |
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