Highly specific antisera against native ovine follitropin (oFSH) obtained from crude antisera by affinity chromatography on solid-phase undissociable lutropin (LH) column
| Autor: | Yves Combarnous, M. R. Blanc, Jean-Claude Poirier |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism Gel matrix Dose-Response Relationship Immunologic Radioimmunoassay Follitropin Cross Reactions Chromatography Affinity Endocrinology Affinity chromatography Antibody Specificity Internal medicine medicine Animals Antiserum chemistry.chemical_classification Chromatography Sheep biology Chemistry Elution Immune Sera General Medicine Luteinizing Hormone biology.protein Rabbits Antibody Follicle Stimulating Hormone Glycoprotein |
| Zdroj: | Acta endocrinologica. 106(1) |
| ISSN: | 0001-5598 |
| Popis: | Antibodies recognizing determinants of the α-subunit, which is common to all glycoprotein hormones, were eliminated from antisera against native oFSH by affinity chromatography. Since the free α-subunit is immunologically different from the α-subunit in the intact hormone, we did not use an α-subunit affinity column. Instead, the antisera were applied to an LH affinity column. However, because intact LH dissociates in the conditions used to elute the purified antibodies, we prepared an LH derivative with covalently-linked subunits and coupled it to gel matrix. By this method oFSH antisera were freed from their non-specific antibodies. The cross-reaction of ovine lutropin in the oFSH radioimmunoassay (RIA) was lowered from 2% to less than 0.1%. Moreover, as the columns can be used repeatedly over long periods with no apparent loss of efficiency, large volumes of antiserum can be treated in this manner. |
| Databáze: | OpenAIRE |
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