Molecular Epidemiology of Multi-Drug Resistant Pseudomonas aeruginosa Isolates from Hospitalized Patients in Greece
| Autor: | Evaggelos Vogiatzakis, Athena Mavridou, Olga Pappa, Eleni Moraitou, Kyriaki Tryfinopoulou, Alex Galanis, Kostas Kostoulas, Chrysa Dioli, Maria Panopoulou, Apostolos Beloukas, Anastasia Maria Kefala, Marios Dimitriou |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) DLST medicine.drug_class 030106 microbiology Antibiotics Locus (genetics) multi drug resistance Biology medicine.disease_cause Microbiology 03 medical and health sciences Virology medicine Typing lcsh:QH301-705.5 Molecular epidemiology Pseudomonas aeruginosa Outbreak Antimicrobial biology.organism_classification FEPR-CAZS 030104 developmental biology P. aeruginosa lcsh:Biology (General) oprD Bacteria HAIs |
| Zdroj: | Microorganisms, Vol 8, Iss 1652, p 1652 (2020) Microorganisms Volume 8 Issue 11 |
| ISSN: | 2076-2607 |
| Popis: | Resistant Pseudomonas aeruginosa isolates are one of the major causes of both hospital-acquired infections (HAIs) and community-acquired infections (CAIs). However, management of P. aeruginosa infections is difficult as the bacterium is inherently resistant to many antibiotics. In this study, a collection of 75 P. aeruginosa clinical isolates from two tertiary hospitals from Athens and Alexnadroupolis in Greece was studied to assess antimicrobial sensitivity and molecular epidemiology. All P. aeruginosa isolates were tested for susceptibility to 11 commonly used antibiotics, and the newly introduced Double Locus Sequence Typing (DLST) scheme was implemented to elucidate the predominant clones. The tested P. aeruginosa isolates presented various resistant phenotypes, with Verona Integron-Mediated Metallo-&beta lactamase (VIM-2) mechanisms being the majority, and a new phenotype, FEPR-CAZS, being reported for the first time in Greek isolates. DLST revealed two predominant types, 32-39 and 8-37, and provided evidence for intra-hospital transmission of the 32-39 clone in one of the hospitals. The results indicate that DLST can be a valuable tool when local outbreaks demand immediate tracking investigation with limited time and financial resources. |
| Databáze: | OpenAIRE |
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