Identification of a new cancer/germline gene, KK-LC-1, encoding an antigen recognized by autologous CTL induced on human lung adenocarcinoma
| Autor: | Takeshi Hanagiri, Mitsuhiro Takenoyama, Kenji Sugio, Kosei Yasumoto, Makiko Mizukami, Tetsuya So, Masakazu Sugaya, Fukuyama Takashi, Yoshinobu Ichiki, Tomoko So |
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| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
DNA Complementary Lung Neoplasms Clone (cell biology) Biology Adenocarcinoma Epitope Epitopes Antigen Antigens Neoplasm Cell Line Tumor medicine Cytotoxic T cell Humans Cloning Molecular Lung cancer Aged HLA-A Antigens Cancer medicine.disease CTL Oncology HLA-B Antigens Immunology Cancer research K562 Cells T-Lymphocytes Cytotoxic |
| Zdroj: | Cancer research. 66(9) |
| ISSN: | 0008-5472 |
| Popis: | The purpose of our present study is to identify a tumor-specific antigen capable of inducing a specific cellular immune response in lung cancer patients. We established a lung adenocarcinoma cell line, designated as F1121L, and induced tumor-specific CTL clone H1 from regional lymph node lymphocytes of patient F1121. CTL clone H1 lysed autologous tumor cells in an HLA-B*1507-restricted manner, but not autologous EBV-B, phytohemagglutinin-blast cells, and K562. The CTL clone also recognized allogeneic HLA-B*1501- or 1507-positive lung cancer cell lines in the HLA-restricted manner. Using the CTL clone, we identified an antigen-coding gene by cDNA expression cloning technique. The gene consisted of 556 bp, including an open reading frame consisted of 113 amino acids, designated as Kita-kyushu lung cancer antigen 1 (KK-LC-1). A 9-mer peptide (KK-LC-176-84; RQKRILVNL) was identified as an epitope peptide. The genomic DNA of this antigen was located in chromosome Xq22. A reverse transcription-PCR analysis revealed that the mRNA of this gene was only expressed in the testis among normal tissues. It was expressed in 9 of 18 (50%) allogeneic non–small-cell lung cancer cell lines and in 40 of 100 (40%) non–small-cell lung cancer tissues. We thus identified a new tumor antigen–coding gene categorized as a cancer/germline gene by an autologous lung cancer and CTL system. The new cancer/germline gene was located in Xq22, which is apparently different from the locations of previously reported cancer/germline genes. (Cancer Res 2006; 66(9): 4922-8) |
| Databáze: | OpenAIRE |
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