The activity and expression of NTPDase is altered in lymphocytes of multiple sclerosis patients
| Autor: | Roberta Schmatz, Gustavo R. Thomé, Maria Rosa Chitolina Schetinger, Naiara Stefanello, Maísa Corrêa, Cíntia Saydelles da Rosa, Luziane Potrich Bellé, Liliane Oliveira, Vera Maria Morsch, Cinthia M. Mazzanti, Margarete Dulce Bagatini, Roselia Maria Spanevello, Maria Beatriz Moretto |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Multiple Sclerosis Adenosine Deaminase Clinical Biochemistry Ficoll Inflammation Biochemistry Gene Expression Regulation Enzymologic Myelin Immune system Adenosine deaminase Adenosine Triphosphate Antigens CD Recurrence medicine Extracellular Humans Lymphocytes Aged biology Multiple sclerosis Biochemistry (medical) Apyrase General Medicine Middle Aged medicine.disease Adenosine Adenosine Diphosphate medicine.anatomical_structure Case-Control Studies Immunology biology.protein Female medicine.symptom medicine.drug |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 411(3-4) |
| ISSN: | 1873-3492 |
| Popis: | Background Multiple sclerosis (MS) is a demyelinating neurological disease, which is presumed to be a consequence of infiltrating lymphocytes that are autoreactive to myelin proteins. ATP and adenosine contribute to fine-tuning immune responses and NTPDase (CD39) and adenosine deaminase (ADA) are important enzymes in the control of the extracellular levels of these molecules at the site of inflammation. We evaluated the activity and expression of NTPDase and adenosine deaminase (ADA) activity in lymphocytes from patients with the relapsing–remitting form of MS (RRMS). Methods This study involved 22 patients with RRMS and 22 healthy subjects as a control group. The lymphocytes were isolated from blood and separated on Ficoll density gradients and after isolation the NTPDase and ADA activities were determined. Results The NTPDase activity and expression were increased in lymphocytes from RRMS patients when compared with the control group (p |
| Databáze: | OpenAIRE |
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