Monitoring of Bacterial Sugars and Hydroxy Fatty Acids in Dust from Air Conditioners by Gas Chromatography-Mass Spectrometry
| Autor: | Alvin Fox, Rose Marie T. Rosario, Lennart Larsson |
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| Rok vydání: | 1993 |
| Předmět: |
Chromatography
Ecology Chemistry Muramic acid Mass spectrometry complex mixtures Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Hydrolysis Environmental and Public Health Microbiology Gas chromatography Peptidoglycan Gas chromatography–mass spectrometry Derivatization Food Science Biotechnology Air filter |
| Zdroj: | Applied and Environmental Microbiology. 59:4354-4360 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.59.12.4354-4360.1993 |
| Popis: | Bacterial levels in dust collected from hospital air-conditioning filters were examined by chemical analysis (without prior culture). The dust was analyzed by gas chromatography-mass spectrometry after hydrolysis and derivatization. l -Glycero- d -mannoheptose and hydroxy fatty acids (3-OH 12:0 and 3-OH 14:0) (primarily found in lipopolysaccharide) and muramic acid (a chemical marker for bacterial peptidoglycan) were present at higher levels in dust collected from filters primarily contacting outdoor (as opposed to indoor) air. The ratio of l -glycero- d -mannoheptose to muramic acid in dust (compared with those of a group of gram-positive and gram-negative bacteria) suggested that both dust types contained appreciable numbers of gram-negative bacteria. There is potential for the chemical assessment of the microbial content of airborne dust. |
| Databáze: | OpenAIRE |
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