Comparison of Bleaching Products With Up to 6% and With More Than 6% Hydrogen Peroxide: Whitening Efficacy Using BI and WID and Side Effects – An in vitro Study
| Autor: | Andreas Schedle, Alexander Franz, Martina Schmid-Schwap, Rinet Dauti, Fabian Kanz, Barbara Cvikl, Hermann Agis, Andreas Moritz, Bledar Lilaj |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
genetic structures
Physiology Scanning electron microscope hydrogen peroxide 02 engineering and technology lcsh:Physiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine stomatognathic system Physiology (medical) In vitro study Viability assay Hydrogen peroxide Cytotoxicity enamel surface morphology Enamel paint lcsh:QP1-981 Sem analysis bleached index 030206 dentistry tooth bleaching 021001 nanoscience & nanotechnology Staining stomatognathic diseases chemistry visual_art SEM visual_art.visual_art_medium cytotoxicity sense organs 0210 nano-technology Nuclear chemistry |
| Zdroj: | Frontiers in Physiology, Vol 10 (2019) |
| DOI: | 10.3389/fphys.2019.00919/full |
| Popis: | The aim of this study was to evaluate the effect of bleaching agents containing different concentrations of hydrogen peroxide (HP) on color-change and on enamel-surface in bovine teeth. Furthermore the influence on cell viability and proliferation was investigated. Two hundred and forty teeth were randomly assigned into four groups (home bleaching ≤6%, in-office bleaching ≤6%, in-office bleaching > 6% HP, and control group). Bleaching was performed after artificial staining and the bleached index (BI) as well as the whiteness index (WID) was measured at several time points. Chemical analysis for HP concentrations and the pH of the bleaching products was done. Furthermore, enamel surfaces of randomly selected specimens were analyzed using scanning electron microscopy (SEM) and cytotoxicity of the tested bleaching products was evaluated in vitro using dental pulp cells (DPCs) and L929 cells. A statistically significant whitening effect was observed in almost all products. As expected all investigated products resulted in decreased cell viability, however, with different values of LC50 (median lethal concentration). SEM analysis showed an analog of enamel alterations with decreasing pH, increasing exposure time, and increasing HP concentration. Bleaching agents containing a low HP concentration are considered to be effective and to have less damaging effects on enamel and tested cells. |
| Databáze: | OpenAIRE |
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