Very long-term self-renewal of small intestine, colon, and hair follicles from cycling Lgr5+ve stem cells
| Autor: | Nick Barker, J H van Es, Hans Clevers, Maria Kasper, Hugo J. Snippert, Viljar Jaks, Rune Toftgård |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Time Factors
Colon Cell Mice Transgenic Biology Biochemistry Models Biological Receptors G-Protein-Coupled Mice Intestine Small Genetics medicine Animals Molecular Biology Cell Proliferation Cell growth Cell Cycle LGR5 Gene Expression Regulation Developmental Cell cycle Hair follicle Intestinal epithelium Small intestine Recombinant Proteins Cell biology Adult Stem Cells medicine.anatomical_structure Stem cell Hair Follicle Hair |
| Zdroj: | Cold Spring Harbor symposia on quantitative biology. 73 |
| ISSN: | 1943-4456 |
| Popis: | The intestinal epithelium and the hair follicle represent examples of rapidly self-renewing tissue in adult mammals. We have recently identified a novel stem cell gene Lgr5 expressed in multiple adult tissues. At the bottoms of crypts in small intestine and colon as well as in hair follicles, Lgr5 marks cycling cells with stem cell properties (Barker et al. 2007; Jaks et al. 2008). Using an inducible Lgr5-Cre knockin allele in conjunction with the Rosa26-LacZ Cre reporter strain, long-term lineage-tracing experiments were performed in adult mice. The Lgr5(+ve) crypt-based cell generated all epithelial lineages during a 14-month period, implying that it represents the stem cell of the small intestine and colon. Similarly, lineage tracing during a 14-month period revealed that Lgr5(+ve) cells located in the bulge of the hair follicle sustained multiple rounds of hair growth. These observations support the counterintuitive notion that Lgr5(+ve) cells are actively cycling, yet represent long-term stem cells of these adult, self-renewing tissues. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|