Development and Characterization of a Benchtop Corneal Puncture Injury Model
| Autor: | Lauren E. Cornell, Brandon M. Gross, Eric J. Snider, Jorge M. Acevedo, Peter R. Edsall, David O. Zamora, Brian J. Lund |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Intraocular pressure medicine.medical_specialty genetic structures Swine Visual Acuity lcsh:Medicine Punctures Article Biomaterials 03 medical and health sciences 0302 clinical medicine Injury Site Battlefield Ophthalmology medicine Animals lcsh:Science Intraocular Pressure Biological models Wound Healing Multidisciplinary Blindness business.industry lcsh:R medicine.disease eye diseases Disease Models Animal 030104 developmental biology 030221 ophthalmology & optometry lcsh:Q Injury model sense organs Visual system business Corneal Injuries |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) |
| ISSN: | 2045-2322 |
| Popis: | During recent military operations, eye-related injuries have risen in frequency due to increased use of explosive weaponry which often result in corneal puncture injuries. These have one of the poorest visual outcomes for wounded soldiers, often resulting in blindness due to the large variations in injury shape, size, and severity. As a result, improved therapeutics are needed which can stabilize the injury site and promote wound healing. Unfortunately, current corneal puncture injury models are not capable of producing irregularly shaped, large, high-speed injuries as seen on the battlefield, making relevant therapeutic development challenging. Here, we present a benchtop corneal puncture injury model for use with enucleated eyes that utilizes a high-speed solenoid device suitable for creating military-relevant injuries. We first established system baselines and ocular performance metrics, standardizing the different aspects of the benchtop model to ensure consistent results and properly account for tissue variability. The benchtop model was evaluated with corneal puncture injury objects up to 4.2 mm in diameter which generated intraocular pressure levels exceeding 1500 mmHg. Overall, the created benchtop model provides an initial platform for better characterizing corneal puncture injuries as seen in a military relevant clinical setting and a realistic approach for assessing potential therapeutics. |
| Databáze: | OpenAIRE |
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