Supplementary Figures 1-8 from The Antigen ASB4 on Cancer Stem Cells Serves as a Target for CTL Immunotherapy of Colorectal Cancer

Autor: Toshihiko Torigoe, Noriyuki Sato, Hiroyoshi Hiratsuka, Akihiro Miyazaki, Ichiro Takemasa, Fumitake Hata, Kunihiko Ishitani, Takeshi Terui, Tomohide Tsukahara, Yoshihiko Hirohashi, Akari Takaya, Yasuhiro Kikuchi, Serina Tokita, Ayumi Hongo, Takayuki Kanaseki, Vitaly Kochin, Sho Miyamoto
Rok vydání: 2023
DOI: 10.1158/2326-6066.22538891.v1
Popis: S1. The surface HLA class I levels on SP and MP clones. S2. ASB4 gene expression is scarce in normal tissues but frequent in CRC tissues. S3. Enrichment of ASB4 gene expression by sphere culture of CRC cell lines. S4. Unsorted SW480 is composed of 1-2% of SP and the other MP cells. S5. SM4 recognized and lyses HCT15 as well as SP-H. S6. Selection of a control CTL clone that does respond to IV9-HLA-A24 complexes. S7. In vivo CTL infiltration into tumor lesions. S8. Silencing ASB4 gene expression does not necessarily result in loss of sphere- and tumor-forming ability in SP-H.
Databáze: OpenAIRE