Direct evidence for toxin production byPseudo-nitzschia plurisecta(Bacillariophyceae) and extension of its distribution area

Autor: Nour Ayache, Virginie Raimbault, Nicolas Chomérat, Amandine M.N. Caruana, Zouher Amzil, Gwenael Bilien, Fabienne Hervé, Michael Retho
Rok vydání: 2019
Předmět:
Zdroj: European Journal Of Phycology (0967-0262) (Informa UK Limited), 2019-10, Vol. 54, N. 4, P. 585-594
ISSN: 1469-4433
0967-0262
DOI: 10.1080/09670262.2019.1620342
Popis: We confirm an amnesic shellfish poisoning risk associated with domoic acid production by the species Pseudo-nitzschia plurisecta and determine the toxin profile, which includes three domoic acid isomers. Although the species was reported once in European waters, along the Spanish Atlantic coast, here we describe and characterize P. plurisecta collected during the first occurrence in French Atlantic waters. Three strains (IFR-PPL-15.1, 15.2 and 15.3) were isolated from French coastal water samples in June 2015. These strains were identified and characterized by microscopic observation, molecular (ITS region sequencing and phylogenetic analysis) and metabolite (toxins and pigments) analyses. The cell shape is lanceolate and the valve contains a central interspace with uniseriate stria of oval-square poroids, each divided into 3–10 sectors. The ITS gene sequence is homologous to the original sequence available for the Spanish strain P. plurisecta. The pigment profile contains chl a, c3, c2, fucoxanthin, diatoxanthin, diadinoxanthin and traces of β,β-carotene. Results on morphology, phylogeny and pigments led to the identification of P. plurisecta and confirmed its first occurrence in French waters.
Databáze: OpenAIRE
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