Epigenetic origin of evolutionary novel centromeres
| Autor: | Pietro D'Addabbo, Nicoletta Archidiacono, Claudia Rita Catacchio, Doron Tolomeo, Evan E. Eichler, Roscoe Stanyon, Werner Schempp, Maika Malig, Oronzo Capozzi, Mariano Rocchi, Stefania Purgato, John Huddleston, Giovanni Perini |
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| Přispěvatelé: | Tolomeo, Doron, Capozzi, Oronzo, Stanyon, Roscoe R., Archidiacono, Nicoletta, D'Addabbo, Pietro, Catacchio, Claudia R., Purgato, Stefania, Perini, Giovanni, Schempp, Werner, Huddleston, John, Malig, Maika, Eichler, Evan E., Rocchi, Mariano |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pongo abelii neocentromere Neocentromere Satellite DNA Centromere polymorphisms of centromeres DNA Satellite Biology Genome Article Cell Line Epigenesis Genetic Evolution Molecular 03 medical and health sciences evolution of centromere Animals Humans Epigenetics Gene Conserved Sequence Chromosome 12 Segmental duplication Multidisciplinary neocentromeres orangutan chromosome rearrangements BAC-FISH 030104 developmental biology Evolutionary biology |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep41980 |
| Popis: | Most evolutionary new centromeres (ENC) are composed of large arrays of satellite DNA and surrounded by segmental duplications. However, the hypothesis is that ENCs are seeded in an anonymous sequence and only over time have acquired the complexity of “normal” centromeres. Up to now evidence to test this hypothesis was lacking. We recently discovered that the well-known polymorphism of orangutan chromosome 12 was due to the presence of an ENC. We sequenced the genome of an orangutan homozygous for the ENC, and we focused our analysis on the comparison of the ENC domain with respect to its wild type counterpart. No significant variations were found. This finding is the first clear evidence that ENC seedings are epigenetic in nature. The compaction of the ENC domain was found significantly higher than the corresponding WT region and, interestingly, the expression of the only gene embedded in the region was significantly repressed. |
| Databáze: | OpenAIRE |
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