Immune profiling with a Salmonella Typhi antigen microarray identifies new diagnostic biomarkers of human typhoid
| Autor: | Philip L. Felgner, Rie Nakajima-Sasaki, Silvia Juarez, Tran Vu Thieu Nga, Li Liang, Stephen Baker, Sarah J. Dunstan, Stephen J. McSorley, D. Huw Davies |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Salmonella
Francisella-Tularensis Proteome 030231 tropical medicine Antibody-Responses Protein Array Analysis Discovery medicine.disease_cause Salmonella typhi Signature Sensitivity and Specificity Immunoglobulin G Typhoid fever Article Microbiology Serology 03 medical and health sciences 0302 clinical medicine Antigen Paratyphoid Fever medicine Medicine and Health Sciences Humans Antigens Cloning Molecular Typhoid Fever Children 030304 developmental biology Protein Microarray 0303 health sciences Multidisciplinary biology Reveals Paratyphoid fever Life Sciences medicine.disease Antibodies Bacterial 3. Good health Immunoglobulin M ROC Curve Vietnam Immunology biology.protein Prediction Biomarkers |
| Zdroj: | Liang, Li; Juarez, Silvia; Nga, Tran Vu Thieu; Dunstan, Sarah; Nakajima-Sasaki, Rie; Davies, D. Huw; et al.(2013). Immune profiling with a Salmonella Typhi antigen microarray identifies new diagnostic biomarkers of human typhoid. Scientific Reports, 3. doi: 10.1038/srep01043. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/0jv2p4v4 Scientific Reports |
| Popis: | Current serological diagnostic assays for typhoid fever are based on detecting antibodies against Salmonella LPS or flagellum, resulting in a high false-positive rate. Here we used a protein microarray containing 2,724 Salmonella enterica serovar Typhi antigens (>63% of proteome) and identified antibodies against 16 IgG antigens and 77 IgM antigens that were differentially reactive among acute typhoid patients and healthy controls. The IgG target antigens produced a sensitivity of 97% and specificity of 80%, whereas the IgM target antigens produced 97% and 91% sensitivity and specificity, respectively. Our analyses indicated certain features such as membrane association, secretion and protein expression were significant enriching features of the reactive antigens. About 72% of the serodiagnostic antigens were within the top 25% of the ranked antigen list using a Naïve bayes classifier. These data provide an important resource for improved diagnostics, therapeutics and vaccine development against an important human pathogen. |
| Databáze: | OpenAIRE |
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