Quantitative determination of DNA bridging efficiency of chromatin proteins

Autor:	Valk, R.A. van der, Qin, L., Moolenaar, G.F., Dame, R.T., Dame R.T.
Přispěvatelé:	Dame R.T.
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Bridging (networking) Immunoprecipitation Chemistry Atomic force microscopy Quantitative determination Chromatin 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Optical tweezers Biophysics DNA Genomic organization
Zdroj:	Methods in Molecular Biology ISBN: 9781493986743 Methods in Molecular Biology, 199-209. New York, NY, U.S.A.: Humana Press STARTPAGE=199;ENDPAGE=209;TITLE=Methods in Molecular Biology
Popis:	DNA looping is important for genome organization in all domains of life. The basis of DNA loop formation is the bridging of two separate DNA double helices. Detecting DNA bridge formation generally involves the use of complex single-molecule techniques (atomic force microscopy, magnetic, or optical tweezers). Although DNA bridging can be qualitatively described, quantification of DNA bridging and bridging dynamics using these techniques is challenging. Here, we describe a novel biochemical assay capable of not only detecting DNA bridge formation, but also allowing for quantification of DNA bridging efficiency and the effects of physico-chemical conditions on DNA bridge formation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::423921d5f95d5abd5fc598d8ab9828c6 https://hdl.handle.net/1887/72500 Zobrazit plný text záznamu