Extraction and bioinformatics analysis of Chlamydia trachomatis LpxA
| Autor: | Huan-Huan Feng, Jin-Qiang Yu, Fang Yang, Ying-Tao Mu, De-Kun Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Genetics
clone (Java method) Signal peptide business.industry Computational Biology Chlamydia trachomatis Sequence Analysis DNA Molecular cloning medicine.disease_cause Polymerase Chain Reaction Protein tertiary structure DNA sequencing 03 medical and health sciences Ophthalmology 0302 clinical medicine 030221 ophthalmology & optometry Medicine business Gene Protein secondary structure 030217 neurology & neurosurgery Software |
| Zdroj: | International ophthalmology. 41(2) |
| ISSN: | 1573-2630 |
| Popis: | The aim of this study is to clone the LpxA gene of Chlamydia trachomatis and analyze its biological characteristics. Specific primers were designed according to the sequence of Ct LpxA gene. LpxA gene was amplified by PCR and connected to pMD18-T vectors. Positive clones were selected for PCR and DNA sequencing. Finally, bioinformatics software was used to analyze the biological properties of LpxA protein. The total length of LpxA gene was 840 bp, encoding 280 amino acids. LpxA protein has no signal peptide and was located in bacterial cytoplasm. The prediction of secondary structure showed that the α-helix, extended strand, β-turn and random coil accounted for 19.6%, 32.8%, 11.4% and 36%, respectively. According to the prediction of tertiary structure, three identical LpxA molecules constituted homologous trimers. It was predicted that there were 11 B cell epitopes in LpxA. Ct Lpxa gene was cloned, and LpxA protein structure and function were predicted. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink