Zisupton--A Novel Superfamily of DNA Transposable Elements Recently Active in Fish

Autor: Delphine Galiana-Arnoux, Amandine Darras, Astrid Böhne, Manfred Schartl, Cornelia Schmidt, Qingchun Zhou, Jean-Nicolas Volff
Přispěvatelé: Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, University of Würzburg, French Ministry of National Education, Research and Technology, Bundesministerium fur Bildung und Forschung (BMBF), Agence Nationale de la Recherche (ANR), Institut National de la Recherche Agronomique (INRA, departement PHASE), Centre National de la Recherche Scientifique (CNRS), Fondation pour la Recherche Medicale (FRM)
Rok vydání: 2011
Předmět:
Male
0106 biological sciences
transposon
[SDV]Life Sciences [q-bio]
Transposases
Retrotransposon
PLATYFISH XIPHOPHORUS-MACULATUS
MOLECULAR ANALYSIS
01 natural sciences
Genome
Cyprinodontiformes
MULTIPLE SEQUENCE ALIGNMENT
RETROTRANSPOSABLE ELEMENTS
Y Chromosome
Cloning
Molecular
Insertion sequence
MAXIMUM-LIKELIHOOD
Phylogeny
Transposase
Genetics
0303 health sciences
HMGXB3
ROLLING-CIRCLE TRANSPOSONS
Cysteine Endopeptidases
ZINC-FINGER PROTEINS
Horizontal gene transfer
SCF UBIQUITIN-LIGASE
Transposable element
Molecular Sequence Data
SUMO-1 Protein
SIGNAL-TRANSDUCTION
testis
Biology
010603 evolutionary biology
Evolution
Molecular
03 medical and health sciences
Animals
DNA transposon
Amino Acid Sequence
Molecular Biology
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
SEX-DETERMINING REGION
Polymorphism
Genetic
sex chromosomes
Genetic Variation
Sequence Analysis
DNA
Mutagenesis
Insertional
platyfish
Composite transposon
SUMO
Ulp1 protease
DNA Transposable Elements
Sequence Alignment
Zdroj: Molecular Biology and Evolution
Molecular Biology and Evolution, Oxford University Press (OUP), 2012, 29 (2), pp.631-645. ⟨10.1093/molbev/msr208⟩
ISSN: 1537-1719
0737-4038
DOI: 10.1093/molbev/msr208
Popis: Transposable elements are widespread mobile DNA sequences able to integrate into new locations within genomes. Through transposition and recombination, they significantly contribute to genome plasticity and evolution. They can also regulate gene expression and provide regulatory and coding sequences (CDSs) for the evolution of novel gene functions. We have identified a new superfamily of DNA transposon on the Y chromosome of the platyfish Xiphophorus maculatus. This element is 11 kb in length and carries a single CDS of 24 exons. The N-terminal part of the putative protein, which is expressed in all adult tissues tested, contains several nucleic acid- and protein-binding domains and might correspond to a novel type of transposase/integrase not described so far in any transposon. In addition, a testis-specific splice isoform encodes a C-terminal Ulp1 SUMO protease domain, suggesting a function in posttranslational protein modification mediated by SUMO and/or ubiquitin small peptides. Accordingly, this element was called Zisupton, for Zinc finger SUMO protease transposon. Beside the Y-chromosomal sequence, five other very similar copies were identified in the platyfish genome. All copies are delimited by 99-bp conserved subterminal inverted repeats and flanked by copy-specific 8-nt target site duplications reflecting their integration at different positions in the genome. Zisupton elements are inserted at different genomic locations in different poeciliid species but also in different populations of X. maculatus. Such insertion polymorphisms between related species and populations indicate relatively recent transposition activity, with a high degree of nucleotide identity between species suggesting possible implication of horizontal gene transfer. Zisupton sequences were detected in other fish species, in urochordates, cephalochordates, and hemichordates as well as in more distant organisms, such as basidiomycete fungi, filamentous brown algae, and green algae. Possible examples of nuclear genes derived from Zisupton have been identified. To conclude, our analysis has uncovered a new superfamily of DNA transposons with potential roles in genome diversity and evolutionary innovation in fish and other organisms.
Databáze: OpenAIRE
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