King Cobra (Ophiophagus hannah) Venom L-Amino Acid Oxidase Induces Apoptosis in PC-3 Cells and Suppresses PC-3 Solid Tumor Growth in a Tumor Xenograft Mouse Model
| Autor: | Ivy Chung, Nget Hong Tan, Mui Li Lee, Jayalakshmi Pailoor, Swee Hung Cheah, Shin Yee Fung |
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| Rok vydání: | 2014 |
| Předmět: |
Male
anti-proliferative King cobra King Cobra Venom NU/NU mice Apoptosis L-Amino Acid Oxidase Mice Annexin In vivo Animals Humans anti-PC-3 tumor Cytotoxic T cell Elapidae Cytotoxicity Cell Proliferation biology Ophiophagus hannah Prostatic Neoplasms General Medicine biology.organism_classification Xenograft Model Antitumor Assays Molecular biology In vitro Immunology Snake Venoms Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| DOI: | 10.7150/ijms.8096 |
| Popis: | King cobra (Ophiophagus hannah) venom L-amino acid oxidase (OH-LAAO), a heat stable enzyme, has been shown to exhibit very potent anti-proliferative activity against human breast and lung tumorigenic cells but not in their non-tumorigenic counterparts. We further examine its in vitro and in vivo anti-tumor activity in a human prostate adenocarcinoma (PC-3) model. OH-LAAO demonstrated potent cytotoxicity against PC-3 cells with IC50 of 0.05 µg/mL after 72 h incubation in vitro. It induced apoptosis as evidenced with an increase in caspase-3/7 cleavages and an increase in annexin V-stained cells. To examine its in vivo anti-tumor activity, we treated PC-3 tumor xenograft implanted subcutaneously in immunodeficient NU/NU (nude) mice with 1 µg/g OH-LAAO given intraperitoneally (i.p.). After 8 weeks of treatment, OH-LAAO treated PC-3 tumors were markedly inhibited, when compared to the control group (P |
| Databáze: | OpenAIRE |
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