IL-11 up-regulates Tie-2 expression during the healing of gastric ulcers in rats
| Autor: | Kazuko Shichijo, Masahiro Ito, Zhao-Min Xu, Masahiro Nakashima, Toshiyuki Nakayama, Hui Wang, Long-Dian Chen, Mutsumi Matsuu, Chun-Yang Wen, Ichiro Sekine |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Male
Pathology medicine.medical_specialty Angiogenesis Pharmacology Western blot medicine Animals Humans Stomach Ulcer Bovine serum albumin Wound Healing biology medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Stomach Gastroenterology Receptor Protein-Tyrosine Kinases General Medicine Immunohistochemistry Receptor TIE-2 digestive system diseases Recombinant Proteins Rats Blot Reverse transcription polymerase chain reaction Disease Models Animal medicine.anatomical_structure Basic Research Gene Expression Regulation biology.protein Wound healing Interleukin-1 |
| Popis: | AIM: To investigate Tie-2 expression during the repair of acetic acid-induced gastric ulcers in rats treated with recombinant human IL-11 (rhIL-11) and in untreated control animals. METHODS: Gastric ulcers were induced in male Wistar rats by applying acetic acid to the fundus of the stomach. RhIL-11 (100 µg/kg twice daily, subcutaneously) was administered from two days before ulcer induction and continued for five days after the induction. Control rats received bovine serum albumin. Gastric specimens were collected at 3 and 5 d after the induction of ulcer for immunohistochemical observation, Western blotting, and reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Immunohistochemical and Western blot analysis demonstrated that Tie-2 expression was enhanced in the rhIL-11-treated rats compared with the control animals at both intervals. CONCLUSION: These findings suggested that IL-11 could accelerate ulcer healing, in part, by up-regulating Tie-2 expression and promoting angiogenesis. |
| Databáze: | OpenAIRE |
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