Neuronal Injury, Gliosis, and Glial Proliferation in Two Models of Temporal Lobe Epilepsy
| Autor: | E. Jill Dahle, Melissa Barker-Haliski, Karen S. Wilcox, H. Steve White, Jaycie L. Loewen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Hippocampus Nerve Tissue Proteins Hippocampal formation Microgliosis Pathology and Forensic Medicine 03 medical and health sciences Cellular and Molecular Neuroscience Epilepsy Mice 0302 clinical medicine Theilovirus medicine Cardiovirus Infections Kindling Neurologic Animals Gliosis Cell Proliferation Neurons Microscopy Confocal Dentate gyrus Calcium-Binding Proteins Microfilament Proteins General Medicine Original Articles medicine.disease Fluoresceins Astrogliosis nervous system diseases Mice Inbred C57BL Disease Models Animal 030104 developmental biology Ki-67 Antigen Neurology nervous system Epilepsy Temporal Lobe Neurology (clinical) medicine.symptom Neuron death Psychology Neuroscience Neuroglia 030217 neurology & neurosurgery |
| Zdroj: | Journal of neuropathology and experimental neurology. 75(4) |
| ISSN: | 1554-6578 |
| Popis: | It is estimated that 30%-40% of epilepsy patients are refractory to therapy and animal models are useful for the identification of more efficacious therapeutic agents. Various well-characterized syndrome-specific models are needed to assess their relevance to human seizure disorders and their validity for testing potential therapies. The corneal kindled mouse model of temporal lobe epilepsy (TLE) allows for the rapid screening of investigational compounds, but there is a lack of information as to the specific inflammatory pathology in this model. Similarly, the Theiler murine encephalomyelitis virus (TMEV) model of TLE may prove to be useful for screening, but quantitative assessment of hippocampal pathology is also lacking. We used immunohistochemistry to characterize and quantitate acute neuronal injury and inflammatory features in dorsal CA1 and dentate gyrus regions and in the directly overlying posterior parietal cortex at 2 time points in each of these TLE models. Corneal kindled mice were observed to have astrogliosis, but not microgliosis or neuron cell death. In contrast, TMEV-injected mice had astrogliosis, microgliosis, neuron death, and astrocyte and microglial proliferation. Our results suggest that these 2 animal models might be appropriate for evaluation of distinct therapies for TLE. |
| Databáze: | OpenAIRE |
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