Antibacterial and antioxidant properties of mixed linkage beta-oligosaccharides from extracted β-glucan hydrolysed by Penicillium occitanis EGL lichenase
| Autor: | Lilia Belghith-Fendri, Semia Ellouz-Chaabouni, Raoudha Ghorbel, Fatma Chaari, Soumaya Zaouri-Ellouzi, Monia Blibech, Yosra Mehdi, Dorra Driss, Fatma Bouaziz, Fatma Kallel |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
beta-Glucans Antioxidant Glycoside Hydrolases DPPH medicine.medical_treatment Flour alpha-Tocopherol Oligosaccharides Microbial Sensitivity Tests Plant Science 01 natural sciences Biochemistry Antioxidants Hydrolysate Analytical Chemistry Hydrolysis chemistry.chemical_compound 0404 agricultural biotechnology 010608 biotechnology Spectroscopy Fourier Transform Infrared Carbohydrate Conformation medicine Glucan chemistry.chemical_classification Chromatography Chemistry Organic Chemistry Penicillium Barley flour Hordeum 04 agricultural and veterinary sciences Penicillium occitanis 040401 food science Anti-Bacterial Agents Microscopy Electron Scanning Antibacterial activity |
| Zdroj: | Natural Product Research. 30:1353-1359 |
| ISSN: | 1478-6427 1478-6419 |
| DOI: | 10.1080/14786419.2015.1056185 |
| Popis: | The aim of this study was first to ascertain the chemical composition and the physicochemical properties of cereal extracted β-glucan from barley flour. Secondly, to assess the antioxidant properties and the antibacterial properties of extracted β-glucan hydrolysates. The proximate composition, FT-IR and scanning electron microscopy of extracted β-Glucan were studied. Hydrolysates from extracted β-glucan, obtained by lichenase EGL from Penicillium occitanis, were a mixed linkage beta-oligosaccharides (MLBO) of trisaccharides and tetrasaccharides. MLBO showed a DPPH radical scavenger with IC50 about 1.8 ± 0.01 mg/mL whereas the IC50 of extracted β-glucan was about 5 ± 0.01 mg/mL. MLBO showed a high antioxidative capacity (175 μmol/mL α-tocopherol equivalents) at 5 mg/mL. The antimicrobial activity was confirmed against all tested bacteria especially at 20 mg/mL of MLBO while no inhibition was observed for all the strains used after the addition of either EGL or extracted β-glucan. |
| Databáze: | OpenAIRE |
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