Effect of different amino acids and vitamins on lepidopteran cell cultures
| Autor: | G. Fediere, X. Lery |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
chemistry.chemical_classification
Cell growth Cell CELLULE ACIDE AMINE CULTURE CELLULAIRE Stimulation Biology Amino acid VITAMINE chemistry.chemical_compound medicine.anatomical_structure chemistry Biochemistry Cell culture medicine INSECTE NUISIBLE Composition (visual arts) LUTTE BIOLOGIQUE MILIEU DE CULTURE Ecology Evolution Behavior and Systematics Fetal bovine serum Choline chloride |
| Popis: | The composition of the medium is an important factor in the regulation of cell growth and adherence. We have attempted to stimulate those properties by adding some amino acids or vitamins to the culture medium. The results obtained on the Choristoneura fuinerunu (Lepidoptera, Tortricidae) cell line show that the augmentation of the concentration of folic acid (X 10) increases the cell adherence (only O. 15 x lo6 supernatant cells) and stimulates their multiplication (more than 20%). The same results are obtained with a mixture containing high concentrations of all the Grace’ vitamins. Choline chloride does not modify cell behavior. An amino acid mixture only stimulates adherence. The stimulation of the production of adhesion factors represents a first stage in the elimination of fetal bovine serum from media, which ordinarily supply those factors. The mass production of cells at a cheaper cost and the multiplication of insect viruses could permit the replacement of chemical agents for biological control. O 1990 Academic Press, Inc. |
| Databáze: | OpenAIRE |
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