Photodegradation of Lipofuscin in Suspension and in ARPE-19 cells and the Similarity of Fluorescence of the Photodegradation Product with Oxidized Docosahexaenoate
| Autor: | Malgorzata Rozanowska, Bartosz Różanowski |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
retina
Docosahexaenoic Acids Light genetic structures QH301-705.5 Cell Survival retinal pigment epithelium lipofuscin docosahexaenoate docosahexaenoic acid fluorescence photodegradation photobleaching cell viability endocytic activity Article Catalysis Cell Line Lipofuscin Inorganic Chemistry biophysics Humans Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy Photolysis Organic Chemistry General Medicine Endocytosis eye diseases Computer Science Applications Chemistry Microscopy Fluorescence lipids (amino acids peptides and proteins) sense organs Oxidation-Reduction |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 23; Issue 2; Pages: 922 International Journal of Molecular Sciences, Vol 23, Iss 922, p 922 (2022) |
| ISSN: | 1422-0067 |
| DOI: | 10.20944/preprints202112.0228.v1 |
| Popis: | Retinal lipofuscin accumulates with age in the retinal pigment epithelium (RPE), where its fluorescence properties are used to assess retinal health. It was observed that there is a decrease in lipofuscin fluorescence above the age of 75 years and in the early stages of age-related macular degeneration (AMD). The purpose of this study was to investigate the response of lipofuscin isolated from human RPE and lipofuscin-laden cells to visible light, and to determine whether an abundant component of lipofuscin, docosahexaenoate (DHA), can contribute to lipofuscin fluorescence upon oxidation. Exposure of lipofuscin to visible light leads to a decrease in its long-wavelength fluorescence at about 610 nm, with a concomitant increase in the short-wavelength fluorescence. The emission spectrum of photodegraded lipofuscin exhibits similarity with that of oxidized DHA. Exposure of lipofuscin-laden cells to light leads to a loss of lipofuscin granules from cells, while retaining cell viability. The spectral changes in fluorescence in lipofuscin-laden cells resemble those seen during photodegradation of isolated lipofuscin. Our results demonstrate that fluorescence emission spectra, together with quantitation of the intensity of long-wavelength fluorescence, can serve as a marker useful for lipofuscin quantification and for monitoring its oxidation, and hence useful for screening the retina for increased oxidative damage and early AMD-related changes. |
| Databáze: | OpenAIRE |
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