Label-free identification of Erythropoietin isoforms by surface enhanced Raman spectroscopy
| Autor: | Emad L. Izake, Waleed A. Hassanain, Frederick L. Theiss |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Gene isoform
chemistry.chemical_classification Chromatography Chemistry Biomolecule Reproducibility of Results Substrate (chemistry) Surface-enhanced Raman spectroscopy Spectrum Analysis Raman Recombinant Proteins Analytical Chemistry law.invention Extractor law Erythropoietin Recombinant DNA medicine Humans Protein Isoforms Label free medicine.drug |
| Zdroj: | Talanta. 236:122879 |
| ISSN: | 0039-9140 |
| Popis: | We present a sensitive label-free surface enhanced Raman spectroscopy (SERS) method for the discrimination between the recombinant and endogenous human Erythropoietin (EPO) isoforms. The proposed methodology comprises a lectin-functionalised extractor chip for the extraction of the recombinant human EPO (rhuEPO) and the endogenous EPO (enEPO) from blood plasma. The disulfide bond molecular structure of the purified isoforms was modified to chemisorb the biomolecules onto a SERS substrate in a unified orientation, thus maximizing the reproducibility and sensitivity of the SERS measurements. The acquired SERS spectra of the EPO isoforms showed diagnostic Raman bands that allowed for the discrimination between rhuEPO and enEPO. The method was also used for the SERS quantification of rhuEPO and enEPO down to 0.1 pM and 0.5 pM, respectively. The SERS determination of the protein isoforms was cross validated against ELISA. The new SERS method has strong potential for the rapid screening of rhuEPO doping in athletes and for the therapeutic drug monitoring of rhuEPO treatment in cancer patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect