A combination of MTAP and BAP1 immunohistochemistry is effective for distinguishing sarcomatoid mesothelioma from fibrous pleuritis
| Autor: | Masayo Yoshimura, Tohru Tsujimura, Kazuki Nabeshima, Fumiaki Kato, Akinori Iwasaki, Satoshi Makihata, Shinji Matsumoto, Hitoshi Ueda, Ayuko Sato, Yoshiaki Kinoshita, Makoto Hamasaki |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Mesothelioma 0301 basic medicine Pulmonary and Respiratory Medicine Cancer Research Pathology medicine.medical_specialty Lung Neoplasms Pleural Neoplasms In situ hybridization Sensitivity and Specificity Diagnosis Differential 03 medical and health sciences 0302 clinical medicine Humans Medicine Pleurisy Aged Aged 80 and over BAP1 Pleural mesothelioma business.industry Tumor Suppressor Proteins Mesothelioma Malignant Sarcoma Middle Aged Sarcomatoid Mesothelioma Fibrosis Immunohistochemistry Methylthioadenosine phosphorylase 030104 developmental biology Purine-Nucleoside Phosphorylase Oncology 030220 oncology & carcinogenesis Chromosomal region Fish Female business Ubiquitin Thiolesterase |
| Zdroj: | Lung Cancer. 125:198-204 |
| ISSN: | 0169-5002 |
| DOI: | 10.1016/j.lungcan.2018.09.019 |
| Popis: | Objectives Histologic diagnosis of malignant pleural mesothelioma (MPM) is not always straightforward. Loss of BRCA1-associated protein 1 (BAP1) expression as detected by immunohistochemistry (IHC) (BAP1 IHC) and homozygous deletion (HD) of 9p21 as detected by fluorescencein situ hybridization (FISH) (9p21 FISH) are effective for distinguishing malignant mesothelial proliferation from benign proliferation. We have previously reported that immunohistochemical expression of the protein product of the methylthioadenosine phosphorylase (MTAP) gene, which is localized in the 9p21 chromosomal region, is correlated with the deletion status of 9p21 FISH in MPM tissues. In this study, we investigated whether a combination of MTAP and BAP1 IHC could distinguish sarcomatoid MPM from fibrous pleuritis. Materials and Methods We examined IHC expressions of MTAP and BAP1 and 9p21 FISH in sarcomatoid/desmoplastic (n = 18) and biphasic MPM (n = 12) and in fibrous pleuritis (n = 17). In biphasic MPM, only sarcomatoid components were evaluated for IHC and FISH. The sensitivity and specificity of each detection assay for discriminating MPM cases from fibrous pleuritis was determined. In addition, we compared the IHC expression of MTAP with the deletion status of 9p21 FISH. Results MTAP IHC and BAP1 IHC showed 80% and 36.7% sensitivity, respectively, and both showed 100% specificity in differentiating MPM from fibrous pleuritis. A combination of MTAP and BAP1 IHC yielded greater sensitivity (90%) than that detected for MTAP IHC alone or BAP1 IHC alone. Moreover, a high degree of concordance was observed between the results of MTAP IHC and HD of 9p21 FISH (κ = 0.63). Conclusions With an accurate interpretation of results, combined MTAP and BAP1 IHC is a reliable and effective method for distinguishing sarcomatoid MPM from fibrous pleuritis. |
| Databáze: | OpenAIRE |
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