Effect of nimesulide on the growth of human laryngeal squamous cell carcinoma
| Autor: | Liang Jiang, Gang Qin, Chong Zhao, Jinbo Liu, Leiji Li, Zhuoping Liang, Haiyang Wang |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Oncology
medicine.medical_specialty Survivin Blotting Western Apoptosis Real-Time Polymerase Chain Reaction Inhibitor of Apoptosis Proteins Flow cytometry Mice Cell Line Tumor Proliferating Cell Nuclear Antigen Internal medicine medicine Animals Humans Cyclooxygenase Inhibitors MTT assay RNA Messenger Laryngeal Neoplasms Cell Proliferation Mice Inbred BALB C Sulfonamides biology medicine.diagnostic_test business.industry Cell growth Cell Cycle Neoplasms Experimental Cell cycle Flow Cytometry Immunohistochemistry Proliferating cell nuclear antigen Gene Expression Regulation Neoplastic Repressor Proteins Otorhinolaryngology Cyclooxygenase 2 Carcinoma Squamous Cell Cancer research biology.protein business Platelet Aggregation Inhibitors |
| Zdroj: | American Journal of Otolaryngology. 35:120-129 |
| ISSN: | 0196-0709 |
| Popis: | Purpose To investigate the effect of nimesulide on the growth of human laryngeal squamous cell carcinoma. Materials and methods The effect of NIM on Hep-2 cell proliferation was measured by the MTT assay. Flow cytometry was used to evaluate the cell cycle and apoptosis in Hep-2 cells. A Western blot analysis was used to detect changes in the protein expression levels of COX-2, Survivin and proliferating cell nuclear antigen (PCNA) in Hep-2 cells. A Hep-2 tumor xenograft model was established in nude mice to observe tumor growth. The changes in the xenograft tumors were observed after hematoxylin/eosin staining. The expression levels of COX-2, Survivin and PCNA proteins and mRNA were measured by immunohistochemical analysis and RT-PCR, respectively. Results NIM had time- and dose-dependent inhibitory effect on the proliferation of Hep-2 cells. NIM could prevent the progression of the cell cycle. After NIM treatment, COX-2, Survivin and PCNA protein levels were reduced in the Hep-2 cells. The volume and weight of the xenograft tumors in the NIM treatment group were significantly reduced. The NIM treatment group also exhibited significantly reduced expression levels of COX-2, Survivin and PCNA at both the protein and mRNA levels. Conclusions Our results suggested that NIM has significant inhibitory effects on the growth of Hep-2 cells and xenograft tumors in nude mice. Selective COX-2 inhibitors could potentially become part of a comprehensive treatment for laryngeal squamous cell carcinoma. Additional research and development will provide new and broader prospects for the prevention and treatment of laryngeal squamous cell carcinoma. |
| Databáze: | OpenAIRE |
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