Anthocyanins Promote Learning through Modulation of Synaptic Plasticity Related Proteins in an Animal Model of Ageing
| Autor: | Matthew G. Pontifex, Alfonsina D'Amato, Laurie T. Butler, Michael Müller, Pierre Waffo-Teguo, Tristan Richard, Emily Connell, Jeremy P. E. Spencer, Jean-Michel Mérillon, Claire M. Williams, David Vauzour, Catarina Rendeiro |
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| Přispěvatelé: | Norwich Medical School, University of East Anglia [Norwich] (UEA), University of Birmingham [Birmingham], University of Milan, Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Anglia Ruskin University (ARU), University of Reading (UOR) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
cognition Physiology brain Clinical Biochemistry RM1-950 Biology Biochemistry Article 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Neurotrophic factors signalling Molecular Biology Protein kinase B PI3K/AKT/mTOR pathway Arc (protein) Tyrosine hydroxylase Kinase Cell Biology Cell biology 030104 developmental biology Synaptic plasticity [SDE]Environmental Sciences flavonoids neuroprotection Therapeutics. Pharmacology 030217 neurology & neurosurgery |
| Zdroj: | Antioxidants Antioxidants, MDPI, 2021, 10 (8), pp.1-14. ⟨10.3390/antiox10081235⟩ Volume 10 Issue 8 Antioxidants, Vol 10, Iss 1235, p 1235 (2021) |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox10081235⟩ |
| Popis: | International audience; Anthocyanin-rich foods, such as berries, reportedly ameliorate age-related cognitive deficits in both animals and humans. Despite this, investigation into the mechanisms which underpin anthocyanin-mediated learning and memory benefits remains relatively limited. The present study investigates the effects of anthocyanin intake on a spatial working memory paradigm, assessed via the cross-maze apparatus, and relates behavioural test performance to underlying molecular mechanisms. Six-week supplementation with pure anthocyanins (2% w/w), administered throughout the learning phase of the task, improved both spatial and psychomotor performances in aged rats. Behavioural outputs were accompanied by changes in the expression profile of key proteins integral to synaptic function/maintenance, with upregulation of dystrophin, protein kinase B (PKB/Akt) and tyrosine hydroxylase, and downregulation of apoptotic proteins B-cell lymphoma-extra-large (Bcl-xL) and the phosphorylated rapidly accelerated fibrosarcoma (p-Raf). Separate immunoblot analysis supported these observations, indicating increased activation of extracellular signal-related kinase (ERK1), Akt Ser473, mammalian target of rapamycin (mTOR) Ser2448, activity-regulated cytoskeleton-associated protein (Arc/Arg 3.1) and brain-derived neurotrophic factor (BDNF) in response to anthocyanin treatment, whilst alpha-E-catenin, c-Jun N-terminal kinase (JNK1) and p38 protein levels decreased. Together, these findings suggest that purified anthocyanin consumption enhances spatial learning and motor coordination in aged animals and can be attributed to the modulation of key synaptic proteins, which support integrity and maintenance of synaptic function. |
| Databáze: | OpenAIRE |
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