Interaction of Late Apoptotic and Necrotic Cells with Vitronectin

Autor: Petr Man, Pavla Angelisova, Ondrej Stepanek, Ondrej Horvath, Jiri Spicka, Vaclav Horejsi, Petr Stockbauer, Tomas Brdicka
Rok vydání: 2011
Předmět:
Erythrocytes
Anatomy and Physiology
Glycobiology
lcsh:Medicine
Apoptosis
Biochemistry
Mass Spectrometry
Extracellular matrix
Jurkat Cells
Mice
Immune Physiology
Molecular Cell Biology
Pathology
lcsh:Science
Cells
Cultured
Extracellular Matrix Proteins
Microscopy
Confocal
Multidisciplinary
Cell Death
biology
medicine.diagnostic_test
Flow Cytometry
Extracellular Matrix
Cell biology
Cytochemistry
Medicine
Vitronectin
biological phenomena
cell phenomena
and immunity
Antibody
Molecular Pathology
Intracellular
Protein Binding
Research Article
Thymus Gland
Antibodies
Cell Line
Flow cytometry
Necrosis
Antigen
Diagnostic Medicine
medicine
Animals
Humans
Antigens
Protein Interactions
Biology
Glycoproteins
Plasma Proteins
lcsh:R
Proteins
Molecular biology
Extracellular Matrix Composition
body regions
Cell culture
biology.protein
lcsh:Q
Wound healing
Spleen
Cytometry
General Pathology
Zdroj: PLoS ONE, Vol 6, Iss 5, p e19243 (2011)
PLoS ONE
ISSN: 1932-6203
Popis: Background Vitronectin is an abundant plasma glycoprotein identified also as a part of extracellular matrix. Vitronectin is substantially enriched at sites of injured, fibrosing, inflamed, and tumor tissues where it is believed to be involved in wound healing and tissue remodeling. Little is known about the mechanism of vitronectin localization into the damaged tissues. Methodology/Principal Findings 2E12 antibody has been described to bind a subset of late apoptotic cells. Using immunoisolation followed by mass spectrometry, we identified the antigen recognized by 2E12 antibody as vitronectin. Based on flow cytometry, we described that vitronectin binds to the late apoptotic and necrotic cells in cell cultures in vitro as well as in murine thymus and spleen in vivo. Confocal microscopy revealed that vitronectin binds to an intracellular cytoplasmic structure after the membrane rupture. Conclusions/Significance We propose that vitronectin could serve as a marker of membrane disruption in necrosis and apoptosis for flow cytometry analysis. Moreover, we suggest that vitronectin binding to dead cells may represent one of the mechanisms of vitronectin incorporation into the injured tissues.
Databáze: OpenAIRE
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