Azlocillin can be the potential drug candidate against drug-tolerant Borrelia burgdorferi sensu stricto JLB31
| Autor: | Brindha Sridharan, Venkata Vamsee Aditya Mallajosyula, Mohammed Inayathullah, Mohamed Sohail Ahmed, Hari Hara Potula, Venkata Raveendra Pothineni, Jayakumar Rajadas, Aditya Ambati |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cefotaxime Antibiotics Drug Resistance Drug Evaluation Preclinical lcsh:Medicine Drug resistance Mice Lyme disease lcsh:Science Doxycycline Lyme Disease Mice Inbred C3H Multidisciplinary biology Bacterial Inbred C3H Preclinical Anti-Bacterial Agents Infectious Diseases 5.1 Pharmaceuticals 6.1 Pharmaceuticals Lyme disease microbiology Female Development of treatments and therapeutic interventions Infection Biotechnology medicine.drug medicine.drug_class 030106 microbiology Azlocillin Article 03 medical and health sciences Drug Resistance Bacterial medicine Animals Humans Borrelia burgdorferi Animal business.industry lcsh:R Evaluation of treatments and therapeutic interventions Bacterial pathogenesis bacterial infections and mycoses biology.organism_classification medicine.disease Vector-Borne Diseases Disease Models Animal Emerging Infectious Diseases 030104 developmental biology Disease Models Immunology Drug Evaluation lcsh:Q business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) Scientific reports, vol 10, iss 1 |
| ISSN: | 2045-2322 |
| Popis: | Lyme disease is one of most common vector-borne diseases, reporting more than 300,000 cases annually in the United States. Treating Lyme disease during its initial stages with traditional tetracycline antibiotics is effective. However, 10–20% of patients treated with antibiotic therapy still shows prolonged symptoms of fatigue, musculoskeletal pain, and perceived cognitive impairment. When these symptoms persists for more than 6 months to years after completing conventional antibiotics treatment are called post-treatment Lyme disease syndrome (PTLDS). Though the exact reason for the prolongation of post treatment symptoms are not known, the growing evidence from recent studies suggests it might be due to the existence of drug-tolerant persisters. In order to identify effective drug molecules that kill drug-tolerant borrelia we have tested two antibiotics, azlocillin and cefotaxime that were identified by us earlier. The in vitro efficacy studies of azlocillin and cefotaxime on drug-tolerant persisters were done by semisolid plating method. The results obtained were compared with one of the currently prescribed antibiotic doxycycline. We found that azlocillin completely kills late log phase and 7–10 days old stationary phase B. burgdorferi. Our results also demonstrate that azlocillin and cefotaxime can effectively kill in vitro doxycycline-tolerant B. burgdorferi. Moreover, the combination drug treatment of azlocillin and cefotaxime effectively killed doxycycline-tolerant B. burgdorferi. Furthermore, when tested in vivo, azlocillin has shown good efficacy against B. burgdorferi in mice model. These seminal findings strongly suggests that azlocillin can be effective in treating B. burgdorferi sensu stricto JLB31 infection and furthermore in depth research is necessary to evaluate its potential use for Lyme disease therapy. |
| Databáze: | OpenAIRE |
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