Simple method for the quantitative examination of extra column band broadening in microchromatographic systems
| Autor: | Stephen G. Weber, Kathleen E. Schaefer, Amy T. Beisler |
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| Rok vydání: | 2003 |
| Předmět: |
Chromatography
Chemistry Capillary action Organic Chemistry Electrochemical detector Chromatography liquid General Medicine Electrochemical detection Biochemistry Analytical Chemistry chemistry.chemical_compound Small peak Capillary column Spectrophotometry Ultraviolet Concentration gradient Derivatization Chromatography Liquid |
| Zdroj: | Journal of Chromatography A. 986:247-251 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(02)02018-6 |
| Popis: | In recent years capillary chromatography has gained popularity for trace analyses. Most often UV or electrochemical detection is employed because the small peak volumes make post-column derivatization challenging. We have developed a simple method based on flow injection for determining contributions to peak broadening from post-column reactors. The only requirement for application of our methodology is that diffusion be in the Taylor regime so that radial concentration gradients are relaxed enabling mixing purely by diffusion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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