Cloning and Heterologous Expression of Hematin-Dependent Catalase Produced by Lactobacillus plantarum CNRZ 1228
| Autor: | Charles M. A. P. Franz, Anette Herrmann, Nuha M. K. Yousif, Bernhard Tauscher, Hikmate Abriouel, Joachim Stärke, Wilhelm H. Holzapfel, Agus Wijaya |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Molecular Sequence Data
Heterologous Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Lactobacillus medicine Escherichia coli Animals Cloning Molecular Ecology food and beverages Sequence Analysis DNA biology.organism_classification Physiology and Biotechnology Catalase Meat Products Biochemistry biology.protein Hemin Fermentation Heterologous expression Bacteria Lactobacillus plantarum Enterococcus Food Science Biotechnology |
| Popis: | Lactobacillus plantarum CNRZ 1228 exhibited heme-dependent catalase activity under environmental conditions similar to those encountered during sausage fermentation. The 1,455-bp catalase gene ( katL ) was cloned and encoded a protein of 484 amino acids. Expression of katL in a heterologous host showed that katL encodes a functional catalase. PCR screening of selected strains of lactic acid bacteria for katL indicated the presence of similar genes in other strains of lactobacilli. |
| Databáze: | OpenAIRE |
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