Reduced Antigenicity of Type I Collagen and Proteoglycans in Sclerotic Dentin
| Autor: | Matteo Biasotto, Alessandra Ruggeri, Mirella Falconi, C Prati, D.H. Pashley, Pietro Suppa, Lorenzo Breschi, Fr Tay |
|---|---|
| Přispěvatelé: | Suppa P, Ruggeri A jr, Tay FR, Prati C, Biasotto M, Falconi M, Pashley DH, Breschi L., Suppa, P, RUGGERI A., Jr, Tay, Fr, Prati, C, Biasotto, Matteo, Falconi, M, Pashley, Dh, Breschi, Lorenzo |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
0301 basic medicine
Antigenicity Pathology medicine.medical_specialty Dental Caries Dentin Secondary Article Collagen Type I Collagen fibril 03 medical and health sciences 0302 clinical medicine stomatognathic system Carious teeth Dentin medicine Humans Organic matrix General Dentistry biology Chemistry 030206 dentistry Anatomy Primary and secondary antibodies Immunohistochemistry Dentin Permeability stomatognathic diseases 030104 developmental biology medicine.anatomical_structure biology.protein Microscopy Electron Scanning Proteoglycans Type I collagen |
| Popis: | Antigenic alterations to the dentin organic matrix may be detected by an immunohistochemical approach. We hypothesized that alterations in the antigenicity of type I collagen and proteoglycans occur in sclerotic dentin under caries lesions. Transverse sections were prepared from carious teeth in the sclerotic zone and normal hard dentin. A double-immunolabeling technique was performed on these sections, with anti-type I collagen and anti-chondroitin 4/6 sulfate monoclonal primary antibodies. We used gold-conjugated secondary antibodies to visualize the distribution of intact collagen fibrils and proteoglycans by high-resolution SEM. For sclerotic dentin, labeling densities were 19.57 ± 3.01/μm2 for collagen and 9.84 ± 2.62/μm2 for proteoglycans. For normal hard dentin, values were 35.20 ± 2.73/μm2 and 17.03 ± 1.98/μm2, respectively. Distribution of intact collagen fibrils and proteoglycans in sclerotic dentin was significantly lower than in normal hard dentin. Reductions in antigenicity from the organic matrix of sclerotic dentin under caries lesions raise concern about the potential of intrafibrillar remineralization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|