p53 in rheumatoid arthritis synovial fibroblasts at sites of invasion
| Autor: | C A, Seemayer, S, Kuchen, M, Neidhart, P, Kuenzler, V, Rihosková, E, Neumann, M, Pruschy, W K, Aicher, U, Müller-Ladner, R E, Gay, B A, Michel, G S, Firestein, S, Gay |
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| Rok vydání: | 2003 |
| Předmět: |
Pathology
medicine.medical_specialty Immunology Population Arthritis Mice SCID Immunofluorescence General Biochemistry Genetics and Molecular Biology Arthritis Rheumatoid Mice Rheumatology In vivo Immunology and Allergy Medicine Synovial fluid Animals Humans education Cells Cultured education.field_of_study medicine.diagnostic_test business.industry Cartilage Synovial Membrane Fibroblasts medicine.disease Genes p53 Immunohistochemistry Extended Report medicine.anatomical_structure Gene Expression Regulation Synovial membrane Tumor Suppressor Protein p53 business |
| Zdroj: | Annals of the rheumatic diseases. 62(12) |
| ISSN: | 0003-4967 |
| Popis: | Objective: To analyse the functional response of p53 in rheumatoid arthritis synovial fibroblasts (RASF) in vitro and in vivo and to investigate whether activation of p53 modulates the destructive process of RASF. Methods: RASF and controls grown on chamber slides were either directly examined with DO7 anti-p53 antibodies by immunofluorescence or irradiated with 10 Gy x rays and analysed time dependently for the expression of p53. The percentage of positive cells was evaluated by a quantitative scoring system. RASF and normal (N) SF cultured in vitro were co-implanted with human cartilage in SCID mice for 60 days. Consecutively, the invasion score was evaluated, and the number of p53 positive cells was determined at the sites of invasion by immunohistochemistry. In addition, synovial tissues from RA, osteoarthritis, and normal synovia were stained with DO7 antibodies. Results: In vitro the rate of expression of p53 in RASF was low ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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