MITOCHONDRIA IN BIODOSIMETRY: FLOW CYTOMETRY ASSESMENT IN VITRO

Autor:	Zuzana, Šinkorová, Anna, Lierová, Alžběta, Filipová, Jana, Čížková, Aleš, Tichý, Jaroslav, Pejchal, Marcela, Milanová, Zdena, Vilasová, Lenka, Andrejsová
Rok vydání:	2022
Předmět:	Membrane Potential Mitochondrial Radiation Radiological and Ultrasound Technology Swine Public Health Environmental and Occupational Health Animals Apoptosis Dose-Response Relationship Radiation Radiology Nuclear Medicine and imaging General Medicine Flow Cytometry Mitochondria
Zdroj:	Radiation Protection Dosimetry. 198:521-526
ISSN:	1742-3406 0144-8420
Popis:	The JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. The probe was tested in vitro on two established cell lines and peripheral porcine blood lymphocytes after gamma irradiation (IR) to assess its potential in biodosimetric evaluation. In brief, we stained irradiated and non-irradiated cells with the JC-1 dye to determine the existing changes in mitochondrial membrane potential and monitor cell health through flow cytometry. The stage of injury in these cells was evaluated through an irradiated versus non-irradiated ratio (IVNIR), comparing the relative proportion of polarised cells containing red JC-1 aggregates. We observed a decreasing IVNIR as the radiation dose increased (i.e. 0.5; 1; 2; 4; 6; 8 and 10 Gy), performing the analysis at 4, 8 and 24 h after IR in all the tested cells. The results from the JC1-dye test showed that CD4 T lymphocytes were more sensitive to irradiation than other subpopulations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::421f97c574f3c51739ba724023e4deca https://doi.org/10.1093/rpd/ncac092 Zobrazit plný text záznamu