Characterization of the Brain Family of Aromatic Amine N-Acetyltransferases
| Autor: | M A Namboodiri, S J Gaudet |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
Tryptamine Aromatic amine Acetyltransferases Cell Biology Biology High-performance liquid chromatography Cellular and Molecular Neuroscience chemistry.chemical_compound Enzyme Biochemistry chemistry Affinity chromatography Acetyltransferase Arylalkylamine Molecular Biology |
| Zdroj: | Molecular and Cellular Neuroscience. 4:310-318 |
| ISSN: | 1044-7431 |
| DOI: | 10.1006/mcne.1993.1041 |
| Popis: | Arylamine and arylalkylamine N-acetyltransferase activities in the rat brain were isolated and partially characterized. A total of four N -acetyltransferase activities were identified using a combination of three separation procedures, ammonium sulfate fractionation, methotrexate affinity chromatography, and size-exclusion HPLC. Two of the N -acetyltransferase activities demonstrated preference for arylamines and differential sensitivity to inhibition by methotrexate. The other two activities were characterized as arylalkylamine N-acetyltransferase based on their preference for arylalkylamines such as serotonin and tryptamine. Potential functions of these enzyme activities in the brain are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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