Monosized Polymeric Microspheres Designed for Passive Lung Targeting: Biodistribution and Pharmacokinetics after Intravenous Administration
| Autor: | Monica Agnoletti, Hanne Mørck Nielsen, Cristina Rodríguez-Rodríguez, Katayoun Saatchi, Tullio V F Esposito, Urs O. Häfeli, Sylvia Natalie Kłodzińska |
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| Rok vydání: | 2020 |
| Předmět: |
Biodistribution
medicine.drug_class Antibiotics General Physics and Astronomy 02 engineering and technology Pharmacology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Mice Pharmacokinetics Polylactic Acid-Polyglycolic Acid Copolymer In vivo medicine Animals General Materials Science Tissue Distribution Lactic Acid Lung General Engineering 021001 nanoscience & nanotechnology Controlled release In vitro Microspheres 0104 chemical sciences PLGA medicine.anatomical_structure chemistry Administration Intravenous 0210 nano-technology Polyglycolic Acid |
| Zdroj: | ACS nano. 14(6) |
| ISSN: | 1936-086X |
| Popis: | Local as well as systemic therapy is often used to treat bacterial lung infections. Delivery of antibiotics to the vascular side of infected lung tissue using lung-targeting microspheres (MS) is a good alternative to conventional administration routes, allowing for localized high levels of antibiotics. This delivery route can also complement inhaled antibiotic therapy, especially in the case of compromised lung function. We prepared and characterized monodisperse poly(lactic-co-glycolic acid) (PLGA) MS loaded with levofloxacin using a flow-focusing glass microfluidic chip. In vitro characterization showed that the encapsulated LVX displayed a biphasic controlled release during 5 days and preserved its antibacterial activity. The MS degradation was investigated in vitro by cross-sectioning the MS using a focused ion beam scanning electron microscope and in vivo by histological examination of lung tissue from mice intravenously administered with the MS. The MS showed changes in the surface morphology and internal matrix, whereas the degradation in vivo was 3 times faster than that in vitro. No effect on the viability of endothelial and lung epithelial cells or hemolytic activity was observed. To evaluate the pharmacokinetics and biodistribution of the MS, complete quantitative imaging of the 111indium-labeled PLGA MS was performed in vivo with single-photon emission computed tomography imaging over 10 days. The PLGA MS distributed homogeneously in the lung capillaries. Overall, intravenous administration of 12 μm PLGA MS is suitable for passive lung targeting and pulmonary therapy. |
| Databáze: | OpenAIRE |
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