Induction of specific stress response increases resistance of rat liver allografts to cold ischemia and reperfusion injury
| Autor: | Akio Kawamura, Mitsuko Tanaka, Akira Kakita, Tsuyoshi Takahashi, Seiichiro Tsuchihashi, Tohru Tamaki, Yasushi Uchida, Takashi Kaizu |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Transplantation Conditioning Cell Survival Metalloporphyrins medicine.medical_treatment Intercellular Adhesion Molecule-1 Ischemia Gene Expression Protoporphyrins Liver transplantation Pharmacology Cycloheximide chemistry.chemical_compound Stress Physiological Gene expression medicine Animals Transplantation Homologous Cryopreservation Transplantation Cell adhesion molecule business.industry Drug Synergism medicine.disease Immunohistochemistry Liver Transplantation Rats Liver chemistry Rats Inbred Lew Enzyme Induction Reperfusion Injury Heme Oxygenase (Decyclizing) Reperfusion Disease Susceptibility business Reperfusion injury Heme Oxygenase-1 |
| Zdroj: | Transplant International. 16:396-404 |
| ISSN: | 1432-2277 0934-0874 |
| Popis: | Heme oxygenase-1 (HO-1) has been shown to increase cellular resistance against oxidative injury, but the functional significance of this is currently obscure. We investigated the protective role of HO-1, induced by tin-protoporphyrin IX (SnPP), in attenuating liver transplantation injury. Lewis rats were intraperitoneally treated with saline as control, 50 micro mol/kg of SnPP, or 2 mg/kg of cycloheximide (CHX) before SnPP injection. Gene expression of HO-1 was induced after either treatment with SnPP- or CHX + SnPP instead of saline, whereas HO-1 protein synthesis was enhanced in Kupffer-like dendritic cells of the SnPP-treated group. Following reperfusion of liver grafts preserved for 30 h, there were fewer intercellular adhesion molecule-1-positive cells in SnPP-treated livers, significantly reduced numbers of dead cells, and enhanced graft viability. The present data suggest that increased synthesis of HO-1 protein by SnPP pre-conditioning is linked to the improved liver graft viability through inhibition of inflammatory adhesion molecules. |
| Databáze: | OpenAIRE |
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