Somatostatin-14 influences pituitary–ovarian axis in peripubertal rats
| Autor: | Nataša Ristić, Verica Milošević, Milica Manojlović-Stojanoski, Branko Filipović, Milka Sekulić, Branka Šošić-Jurjević, Nataša Nestorović |
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| Rok vydání: | 2008 |
| Předmět: |
endocrine system
medicine.medical_specialty Histology Serum fsh Somatotropic cell Injections Subcutaneous Period (gene) Ovary Stereology Biology Ovarian Follicle Internal medicine medicine Animals Sexual Maturation Rats Wistar Molecular Biology Body Weight Organ Size Cell Biology Immunohistochemistry Growth hormone secretion Rats Medical Laboratory Technology medicine.anatomical_structure Somatostatin Endocrinology Pituitary Gland Female Folliculogenesis |
| Zdroj: | Histochemistry and Cell Biology. 130:699-708 |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/s00418-008-0442-7 |
| Popis: | The effects of multiple somatostatin (SRIH-14) administration on the pituitary-ovarian axis were examined in peripubertal rats. Female Wistar rats received subcutaneously, two daily doses of 20 microg SRIH-14 per 100 g body weight (b.w.) for five consecutive days (from the 33rd to the 37th day of life). Follicle-stimulating (FSH), luteinizing (LH) and somatotropic (GH) cells were examined by the peroxidase-anti-peroxidase immunocytochemical method. Changes in cell volumes, volume densities and number per unit area (mm(2)) of FSH-, LH- and GH-immunoreactive cells were evaluated by stereology and morphometry. Serum FSH and LH levels were determined by RIA. Ovaries were analyzed by simple point counting of follicles. The volumes and volume densities of FSH-, LH- and GH-immunoreactive cells were significantly decreased while their numbers per mm(2) remained unchanged. SRIH-14 induced a significant decrease in serum FSH and LH levels. In the ovary, SRIH-14 induced an increase in the number of primordial follicles, followed by a reduction in the number of small healthy growing follicles and absence of preovulatory follicles. The number of atretic follicles was unchanged. We concluded that treatment with SRIH-14 during the peripubertal period markedly inhibited pituitary FSH, LH and GH cells. In the ovary, SRIH-14 acted by inhibiting folliculogenesis without affecting atretic processes. |
| Databáze: | OpenAIRE |
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