Floating and Tether-Coupled Adhesion of Bacteria to Hydrophobic and Hydrophilic Surfaces
| Autor: | Henk J. Busscher, Prashant K. Sharma, Jelmer Sjollema, Rebecca van der Westen, Robert Molenaar, Henny C. van der Mei |
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| Přispěvatelé: | Nanobiophysics, Man, Biomaterials and Microbes (MBM), Personalized Healthcare Technology (PHT) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Surface Properties UT-Hybrid-D Total internal reflection microscopy 02 engineering and technology 010402 general chemistry Bacterial Physiological Phenomena 01 natural sciences Vibration Article Bacterial Adhesion Electrochemistry Perpendicular Journal Article Environmental Microbiology Perpendicular distance General Materials Science Spectroscopy biology Strain (chemistry) Bacteria Osmolar Concentration Surfaces and Interfaces Adhesion 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification 0104 chemical sciences Ionic strength Biophysics 0210 nano-technology Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Langmuir Langmuir, 34(17), 4937-4944. American Chemical Society Langmuir, 34(17), 4937-4944. AMER CHEMICAL SOC |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | Models for bacterial adhesion to substratum surfaces all include uncertainty with respect to the (ir)reversibility of adhesion. In a model, based on vibrations exhibited by adhering bacteria parallel to a surface, adhesion was described as a result of reversible binding of multiple bacterial tethers that detach from and successively reattach to a surface, eventually making bacterial adhesion irreversible. Here, we use total internal reflection microscopy to determine whether adhering bacteria also exhibit variations over time in their perpendicular distance above surfaces. Streptococci with fibrillar surface tethers showed perpendicular vibrations with amplitudes of around 5 nm, regardless of surface hydrophobicity. Adhering, nonfibrillated streptococci vibrated with amplitudes around 20 nm above a hydrophobic surface. Amplitudes did not depend on ionic strength for either strain. Calculations of bacterial energies from their distances above the surfaces using the Boltzman equation showed that bacteria with fibrillar tethers vibrated as a harmonic oscillator. The energy of bacteria without fibrillar tethers varied with distance in a comparable fashion as the DLVO (Derjaguin, Landau, Verwey, and Overbeek)-interaction energy. Distance variations above the surface over time of bacteria with fibrillar tethers are suggested to be governed by the harmonic oscillations, allowed by elasticity of the tethers, piercing through the potential energy barrier. Bacteria without fibrillar tethers "float" above a surface in the secondary energy minimum, with their perpendicular displacement restricted by their thermal energy and the width of the secondary minimum. The distinction between "tether-coupled" and "floating" adhesion is new, and may have implications for bacterial detachment strategies. |
| Databáze: | OpenAIRE |
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