Asprin‐loaded strontium‐containing α‐calcium sulphate hemihydrate/nano‐hydroxyapatite composite promotes regeneration of critical bone defects
| Autor: | Haoyang Wan, Mo Jiang, Hanjun Qin, Bin Yu, Qi Yu, Yi Jiang, Jun Yang |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Bone Regeneration osteoconductive Chemical Phenomena aspirin nano‐hydroxyapatite Coprecipitation Composite number chemistry.chemical_element Biocompatible Materials Calcium Calcium Sulfate Bone and Bones 03 medical and health sciences 0302 clinical medicine X-Ray Diffraction Osteogenesis In vivo Osteoclast Spectroscopy Fourier Transform Infrared medicine Animals strontium osteoinductive Strontium bone graft substitute Chemistry Regeneration (biology) Mesenchymal Stem Cells X-Ray Microtomography Original Articles Cell Biology Immunohistochemistry calcium sulphate hemihydrate In vitro Rats Durapatite 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Bone Substitutes Molecular Medicine Original Article Nuclear chemistry |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| DOI: | 10.1111/jcmm.15918 |
| Popis: | Our laboratory originally synthesized strontium(Sr)‐containing α‐calcium sulphate hemihydrate/nano‐hydroxyapatite composite (Sr‐α‐CSH/n‐HA) and demonstrated its ability to repair critical bone defects. This study attempted to incorporate aspirin into it to produce a better bone graft material for critical bone defects. After 5% Sr‐α‐CSH was prepared by coprecipitation and hydrothermal methods, it was mixed with aspirin solution of different concentrations (50 μg/ml, 200 μg/ml, 800 μg/ml and 3200 μg/ml) at a fixed liquid‐solid ratio (0.54 v/w) to obtain aspirin‐loaded Sr‐α‐CSH/n‐HA composite. In vitro experiments were performed on the composite extracts. The tibial defects (3 mm*5 mm) in SD rat model were filled with the composite for 4 weeks and 12 weeks to evaluate its osteogenic capacity in vivo. Our results showed its capability of proliferation, migration and osteogenesis of BMSCs in vitro got improved. In vivo treatment with 800 μg/ml aspirin–loaded Sr‐α‐CSH/n‐HA composite led to significantly more new bone formation in the defects compared with Sr‐α‐CSH/n‐HA composite and significantly promoted the expression of osteogenic‐related genes and inhibited osteoclast activity. In general, our research suggests that aspirin‐loaded Sr‐α‐CSH/n‐HA composite may have a greater capacity of repairing tibial defects in SD rats than simple Sr‐α‐CSH/n‐HA composite. |
| Databáze: | OpenAIRE |
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