The Role of Dimethoate and UV-B on Skin of Wistar Rats
| Autor: | Celina Tizuko Fujiyama Oshima, Marcelo Souza Silva, Daniel Araki Ribeiro, Juliana Pedrosa Korinfsky, Tania Rita Moreno de Oliveira Fernandes, Hélio Plapler, Leonardo Cardili |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
Ultraviolet Rays Hyperkeratosis Cell Apoptosis Caspase 3 Biology medicine.disease_cause Andrology chemistry.chemical_compound Atrophy parasitic diseases medicine Animals Dimethoate Rats Wistar Cell Proliferation Skin technology industry and agriculture General Medicine medicine.disease Immunohistochemistry Acute toxicity Rats medicine.anatomical_structure Oncology chemistry Female Carcinogenesis Biomarkers |
| Zdroj: | Anticancer Research. 39:5179-5184 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.13714 |
| Popis: | BACKGROUND/AIM The pesticide dimethoate (O-dimethyl-S- Nmethylcarbamoylmethyl phosphorodithioate) is able to induce severe acute toxicity in living organisms. The aim of this study was to evaluate the effects of ultraviolet radiation, alone or combined with exposure to dimethoate, on the rat skin. MATERIALS AND METHODS A total of 38 Wistar female rats (Rattus norvegicus albinus), were distributed into four groups: A (n=9) control group, B (n=10) exposed to ultraviolet-B radiation (UV-B), C (n=10) exposed to UV-B followed by application of dimethoate (UV-B+AGRO) and group D (n=9) exposed to dimethoate (AGRO). Histological examination of the tissues, as well as immunohistochemistry for cleaved caspase 3, Ki-67 and COX-2 expression were performed to all groups. RESULTS Animals submitted to UV-B exhibited hyperkeratosis with moderate cell atypia. Regarding exposure to UV-B+AGRO, the animals presented hyperkeratosis and atrophy, whereas in animals exposed to AGRO, only atrophy was noticed. The immunohistochemical results on skin revealed that UVB, AGRO and UVB+AGRO decreased cleaved caspase 3 and Ki-67 expression when compared to the control group (p |
| Databáze: | OpenAIRE |
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