Genetic diversity and biogeography of rhizobia associated with Caragana species in three ecological regions of China
| Autor: | Xue Rui Yan, Su Hua Guan, Yang Li Lu, Wen Xin Chen, Wen Feng Chen, En Tao Wang |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial China Rhizobiaceae Gene Transfer Horizontal Proteome Molecular Sequence Data Caragana N-Acetylglucosaminyltransferases DNA Ribosomal Applied Microbiology and Biotechnology Microbiology Bradyrhizobium Rhizobia Intergenic region Bacterial Proteins RNA Ribosomal 16S DNA Ribosomal Spacer Botany Cluster Analysis Phylogeny Ecology Evolution Behavior and Systematics Genetic diversity Geography biology Ecology Mesorhizobium Genetic Variation food and beverages Sequence Analysis DNA biochemical phenomena metabolism and nutrition biology.organism_classification bacteria Rhizobium Electrophoresis Polyacrylamide Gel |
| Zdroj: | Systematic and Applied Microbiology. 32:351-361 |
| ISSN: | 0723-2020 |
| Popis: | Twenty-two genospecies belonging mainly to Mesorhizobium, and occasionally to Rhizobium and Bradyrhizobium, were defined among the 174 rhizobia strains isolated from Caragana species. Highly similar nodC genes were found in the sole Bradyrhizobium strain and among all the detected Mesorhizobium strains. A clear correlation between rhizobial genospecies and the eco-regions where they were isolated was found using homogeneity analysis. All these results demonstrated that Caragana species had stringently selected the rhizobia symbiotic genotype, but not the genomic background; lateral transfer of symbiotic genes from Mesorhizobium to Bradyrhizobium and among the Mesorhizobium species has happened in the Caragana rhizobia; and biogeography of Caragana rhizobia exists. Furthermore, a combined cluster analysis, based upon the patterns obtained from amplified 16S rRNA gene and 16S-23S intergenic spacer restriction analyses, BOX PCR and SDS-PAGE of proteins, was reported to be an efficient method to define the genospecies. |
| Databáze: | OpenAIRE |
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