Aldehyde dehydrogenase activity helps identify a subpopulation of murine adipose-derived stem cells with enhanced adipogenic and osteogenic differentiation potential
| Autor: | Shotaro Eto, Yasuho Taura, Munekazu Nakaichi, Tomoya Haraguchi, Masato Hiyama, Yu Arikawa, Shimpei Nishikawa, Yoshiki Itoh, Toshie Iseri, Yusuke Sakai, Kazuhito Itamoto, Kenji Tani, Harumichi Itoh |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Histology Aldehyde dehydrogenase Adipose tissue Ribosome Aldehyde dehydrogenase activity Flow cytometry 03 medical and health sciences Adipose-derived stem/stromal cell Genetics medicine Subpopulation Molecular Biology Genetics (clinical) biology medicine.diagnostic_test Chemistry Cell Biology Basic Study Cell biology 030104 developmental biology Adipogenesis biology.protein Stem cell |
| Zdroj: | World Journal of Stem Cells |
| ISSN: | 1948-0210 |
| Popis: | AIM To identify and characterize functionally distinct subpopulation of adipose-derived stem cells (ADSCs). METHODS ADSCs cultured from mouse subcutaneous adipose tissue were sorted fluorescence-activated cell sorter based on aldehyde dehydrogenase (ALDH) activity, a widely used stem cell marker. Differentiation potentials were analyzed by utilizing immunocytofluorescece and its quantitative analysis. RESULTS Approximately 15% of bulk ADSCs showed high ALDH activity in flow cytometric analysis. Although significant difference was not seen in proliferation capacity, the adipogenic and osteogenic differentiation capacity was higher in ALDHHi subpopulations than in ALDHLo. Gene set enrichment analysis revealed that ribosome-related gene sets were enriched in the ALDHHi subpopulation. CONCLUSION High ALDH activity is a useful marker for identifying functionally different subpopulations in murine ADSCs. Additionally, we suggested the importance of ribosome for differentiation of ADSCs by gene set enrichment analysis. |
| Databáze: | OpenAIRE |
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